Sperm released into the urine of adult male frogs, Rana pipiens, after intraperitoneal injection of human chorionic gonadotropin, were used to fertilize freshly ovulated eggs. Repeated spermiations from the same animal were induced with repeated injections of the hormone at intervals of four to 11 days. Individual frogs were induced to spermiate up to four times, with fertile sperm ensuing each time. Development of zygotes produced by this method did not differ from the development of ova fertilized by conventional methods.
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