Peroxisomes: biochemistry, molecular biology and genetic diseases- a video programme for teaching students.

Biochem Educ

University of Burgundy, LBMC, Faculty Gabriel, 6, Boulevard Gabriel, 21000, Dijon, France

Published: May 2000

AI Article Synopsis

  • Peroxisome proliferation in rodent livers serves as a biochemical marker for detecting the toxic effects of various xenobiotics, including specific drugs and herbicides.
  • Research on peroxisomes highlights the collaboration between basic sciences and applications in biomedical and industrial health.
  • A 25-minute educational video showcases techniques for studying peroxisomes, aimed at undergraduate and advanced biology students, covering methods like immunoblotting, mRNA analysis, and cell culture.

Article Abstract

Peroxisome proliferation in rodent liver is a good biochemical marker of toxicology for several classes of xenobiotics, including fibrates; phthalates and adipates; or chlorophenoxy-acetate, a herbicide. Research in peroxisomes provides a good example of the integration of fields related to basic sciences and biomedical and industrial health. A 25min video programme illustrates techniques involved in the characterization of purified peroxisomes (Fig. 1) and membranes, immunoblotting, measurement of proliferation markers, mRNA analysis at the post-transcriptional level, DNA techniques, cell cultures as biological models and computer analysis. It is aimed at undergraduates and non-biochemist advanced students in biology.

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