Objective: An inherited reduction in nephron number has been implicated in the development of salt-sensitive hypertension and end stage renal disease. The Munich Wistar Frömter (MWF) rat represents a genetic model with a 30-50% reduction of nephrons compared with normal rats. MWF rats develop spontaneous hypertension and increased urinary albumin excretion (UAE). We addressed the question whether the inherited defect in this model leads to salt-sensitive hypertension.
Methods: At the age of 6 weeks, we started male and female MWF/Fub rats and salt-resistant Lewis (Lew) reference rats on either a normal NaCl (0.2%) or a high NaCl (8%) diet (n = 8, each group). Systolic blood pressure (SBP) and UAE were measured at 14 weeks.
Results: Under a normal diet, MWF/Fub rats demonstrated significantly elevated SBP compared to Lew rats both in male (165 +/- 2 versus 133 +/- 3 mmHg, P < 0.0001) and female (156 +/- 3 versus 134 +/- 3 mmHg, P < 0.0001) rats. After high NaCl treatment, SBP was significantly higher in both male and female MWF/Fub rats (+55 mmHg and +36 mmHg, P < 0.0001, respectively) compared with MWF/ Fub under a normal diet UAE was also significantly higher in male and female MWF/Fub rats after high NaCl excess (P < 0.0005, respectively). In contrast, both SBP and UAE remained unchanged in response to high NaCl in Lew rats.
Conclusions: Our findings demonstrate that both the hypertension and UAE are sensitive to high NaCl loading in female and male MWF/Fub rats. Thus, an inborn nephron deficit may lead to salt-sensitive hypertension and renal dysfunction.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/00004872-200018060-00017 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Probing the Interaction Mechanisms of Dodecane and Mica/Calcite: Implications for Oil Recovery.
Langmuir
January 2025
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, P. R. China.
Understanding the interfacial interaction mechanisms between oil and minerals is of vital importance in the applications of petroleum production and environmental protection. In this work, the interactions of dodecane with mica and calcite in aqueous media were investigated by using the drop probe technique based on atomic force microscopy. For the dodecane-mica interactions, the electrical double layer (EDL) repulsion dominated in 10 mM NaCl solution, and a higher pH facilitated the detachment of dodecane.
View Article and Find Full Text PDFCharacterization and application of polysaccharides produced by Pseudomonas atacamensis M7D1 for chromium (III) removal from tannery effluent.
Int J Biol Macromol
January 2025
Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran; Modares Environmental Research Institute, Tarbiat Modares University, Tehran, Iran. Electronic address:
The wastewater from various industries contaminated with heavy metals poses significant environmental challenges. Biosorption has emerged as a widely used method for removing heavy metals from industrial wastewater. Pseudomonas atacamensis M7D1 is known to produce polysaccharides, but the potential of its polysaccharides as an adsorbent for heavy metal removal still needs to be explored.
View Article and Find Full Text PDFCreating a Halotolerant Degrader for Efficient Mineralization of p-Nitrophenol-Substituted Organophosphorus Pesticides in High-Saline Wastewater.
Biotechnol Bioeng
January 2025
Department of Biochemistry and Molecular Biology, College of Life Sciences, Nankai University, Tianjin, China.
The bioaugmentation performance is severely reduced in the treatment of high-saline pesticide wastewater because the growth and degradation activity of pesticide degraders are significantly inhibited by high salt concentrations. In this study, a heterologous biodegradation pathway comprising the seven genes mpd/pnpABCDEF responsible for the bioconversion of p-nitrophenol (PNP)-substituted organophosphorus pesticides (OPs) into β-oxoadipate and the genes encoding Vitreoscilla hemoglobin (VHb) and green fluorescent protein (GFP) were integrated into the genome of a salt-tolerant chassis Halomonas cupida J9, to generate a genetically engineered halotolerant degrader J9U-MP. RT-PCR assays demonstrated that the nine exogenous genes are successfully transcribed to mRNA in J9U-MP.
View Article and Find Full Text PDFFurther Characterization of Lipase B from Ustilago maydis Expressed in Pichia pastoris: a Member of the Candida antarctica Lipase B-like Superfamily.
Appl Biochem Biotechnol
January 2025
Unidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C, 45019, Zapopan, Jal, Mexico.
Lipases from the basidiomycete fungus Ustilago maydis are promising but underexplored biocatalysts due to their high homology with Candida antarctica lipases. This study provides a comprehensive characterization of a recombinant CALB-like lipase from U. maydis, expressed in Pichia pastoris (rUMLB), and compares its properties with those of the well-studied recombinant lipase B from C.
View Article and Find Full Text PDFLong-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA1b in the rat small intestine.
PLoS One
January 2025
Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand.
Although long-term high dietary sodium consumption often aggravates hypertension and bone loss, sodium in the intestinal lumen has been known to promote absorption of nutrients and other ions, e.g., glucose and calcium.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!