The results are presented of the study of the properties (morphological, tinctorial, cultural, biochemical) and primary DNA structure of 28 Pragia fontium strains, 18 of which were isolated in the territory of Ukraine and 10 in the territory of Czechia. The scheme of differentiation of genera Pragia, Budvicia and Leminorella is presented. The GC (guanine + cysteine) content of DNA is 47 +/- 1.5 mol.%. The levels of DNA relatedness of P. fontium strains and the type strain (CNCTC Eb11/82) varied from 84 to 95%. The levels of DNA relatedness of P. fontium and Escherichia coli K-12 strain is 3 to 5%.
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