The use of peptide hydrolase (EC 3.4.13.1) from Xanthomonas rubrilineans for synthesis of the antibiotic cephalexin from 7-aminodesacetoxycephalosporanic acid was studied. The optimum conditions for production of cephalexin were determined, and the yield exceeded 80%. A method for monitoring the synthesis of this antibiotic synthesis by means of a conventional amino acid analyzer is proposed.
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Methods Mol Biol
January 2024
Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Estación Experimental Agroindustrial Obispo Colombres (EEAOC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Tucumán, Argentina.
Acidovorax avenae subsp. avenae (Aaa) is the causal agent of red stripe in sugarcane, a disease characterized by two forms: leaf stripe and top rot. Despite the importance of this disease, little is known about Aaa virulence factors (VFs) and their function in the infection process.
View Article and Find Full Text PDFFront Microbiol
September 2023
National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China.
Auxin response factor (ARF) is a critical regulator in the auxin signaling pathway, involved in a variety of plant biological processes. Here, gene members of 24 and 39 were identified in two genomes of clones AP85-441 and Np-X, respectively. Phylogenetic analysis showed that all genes were clustered into four clades, which is identical to those genes in maize () and sorghum ().
View Article and Find Full Text PDFPlants (Basel)
August 2023
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Bacterial panicle blight, bacterial leaf streak, and bacterial brown stripe are common bacterial diseases in rice that represent global threats to stable rice yields. In this study, we used the rice variety HZ, Nekken and their 120 RIL population as experimental materials. Phenotypes of the parents and RILs were quantitatively analyzed after inoculation with , pv.
View Article and Find Full Text PDFMol Plant Microbe Interact
October 2023
Department of Plant Physiology, Institute of Biology, Leipzig University, 04103 Leipzig, Germany.
In recent years subsp. was identified as a major cause of bacterial etiolation and decline (BED) in turfgrasses and has become a growing economical concern for the turfgrass industry. The symptoms of BED resemble those of "bakanae," or foolish seedling disease, of rice (), in which the gibberellins produced by the infecting fungus, , contribute to the symptom development.
View Article and Find Full Text PDFFront Plant Sci
February 2023
National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
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