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Identifying the Molecular Signatures of Organic Matter Leached from Land-Applied Biosolids via 21 T FT-ICR Mass Spectrometry.
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National High Magnetic Field Laboratory Geochemistry Group and Department of Earth, Ocean, and Atmospheric Science, Florida State University, Tallahassee, Florida 32306, United States.
Intensification of wastewater treatment residual (i.e., biosolid) applications to watersheds can alter the amount and composition of organic matter (OM) mobilized into waterways.
View Article and Find Full Text PDFSubstitutes for Industrial Trans Fats in Packaged Foods: A Scoping Review.
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Postgraduate Program in Nutrition, Health Sciences Center, Federal University of Santa Catarina, Florianópolis 88040-370, Brazil.
The World Health Organization recommended the global elimination of industrial trans fats by 2023, leading to a decrease in their use in packaged foods. Nevertheless, a gap remains in the scientific literature regarding the ingredients adopted as substitutes by the food industry. This study aimed to map evidence on substitutes for industrial trans fats in packaged foods, discussing their possible designation in the ingredients lists.
View Article and Find Full Text PDFEvolutionary Novelties in Bacteria and the Missing Backdrop of the Environment.
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Trivedi School of BioSciences and Koita Centre for Digital Health, Ashoka University, Sonipat, India.
Evolutionary novelty has been one of the central themes in the field of evolutionary biology for many years. Structural and functional innovations such as scales in the reptiles, fins in the fishes and mammary glands in the mammals have been the focus of the studies. Insights obtained from these studies have shaped the criterion for the identification of novelty as well as provide the framework for studying novelty.
View Article and Find Full Text PDFChIP-mini: a low-input ChIP-exo protocol for elucidating DNA-binding protein dynamics in intracellular pathogens.
Nucleic Acids Res
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School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea.
Genome-wide identification of binding profiles for DNA-binding proteins from the limited number of intracellular pathogens in infection studies is crucial for understanding virulence and cellular processes but remains challenging, as the current ChIP-exo is designed for high-input bacterial cells (>1010). Here, we developed an optimized ChIP-mini method, a low-input ChIP-exo utilizing a 5,000-fold reduced number of initial bacterial cells and an analysis pipeline, to identify genome-wide binding dynamics of DNA-binding proteins in host-infected pathogens. Applying ChIP-mini to intracellular Salmonella Typhimurium, we identified 642 and 1,837 binding sites of H-NS and RpoD, respectively, elucidating changes in their binding position and binding intensity during infection.
View Article and Find Full Text PDFUnlabelled: Experimental studies have demonstrated that nutritional changes during development can result in phenotypic changes to mammalian cheek teeth. This developmental plasticity of tooth morphology is an example of phenotypic plasticity. Because tooth development occurs through complex interactions between manifold processes, there are many potential mechanisms which can contribute to a tooth's norm of reaction.
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