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Waveguide Evanescent Field Fluorescence Microscopy Images of Osteoblast Cells: The Effect of Trypsin and Image Processing Using TrackMate.
Microsc Res Tech
January 2025
Department of Computer Science, Cihan University, Sulaimaniyah, Kurdistan Region, Iraq.
Waveguide evanescent field fluorescence microscopy (WEFF) is an evanescent-based microscopy that utilizes a confined thin film of light, around 100 nm, to image the plasma membrane of cells attached to a waveguide. Low photobleaching and low background besides its high axial resolution allows time-lapse imaging to investigate changes in cell morphology in the presence or absence of chemical agents. Both large field of view (FOV) and uniform illumination are very important while imaging cell-substrate contacts with an evanescent field.
View Article and Find Full Text PDFQuantification of indocyanine-green-enhanced fluorescence with spectrophotometry (O2C®) in low anterior rectal resection: A prospective study.
Tech Coloproctol
December 2024
Department of Surgery, St. Marienkrankenhaus Siegen, Kampenstr. 51, 57072, Siegen, Germany.
Introduction: Despite spectacular visuals and the seemingly convincing rationale of using indocyanine-green-enhanced fluorescence in assessing bowel perfusion during colorectal resections, a lingering sense of subjectivity remains in the challenge of quantifying this fluorescence. This prospective study analyzed the application of O2C® spectrophotometry to quantify zones of fluorescence on the large bowel during low anterior resection.
Materials And Methods: Patients receiving a low anterior resection for cancer of the mid- and lower rectum were enrolled in this observational prospective study between February 2020 and December 2022.
Polybrominated diphenyl ether flame retardants inhibit growth factor-induced activation of EGFR by binding to its extracellular domain.
Arch Toxicol
December 2024
IUF - Leibniz-Research Institute for Environmental Medicine, Auf'm Hennekamp 50, 40225, Düsseldorf, Germany.
For many years, polybrominated diphenyl ethers (PBDEs) were used as flame retardants in a large number of consumer products. Even though international law meanwhile prohibits the production and usage of PBDEs, these persistent and bioaccumulative chemicals still leak into the environment, and are frequently detected in wildlife and humans. Population-based studies reveal positive correlations between human PBDE exposure and various adverse health effects, emphasizing that a better understanding of the mode of action of these polybrominated chemicals is urgently needed.
View Article and Find Full Text PDFBiocontrol potential of endophytic Bacillus spp. associated to wild solanaceous species and their secondary metabolites against various potato (Solanum tuberosum L.) storage diseases.
Microb Pathog
November 2024
LR21AGR03-Production and Protection for a Sustainable Horticulture (2PHD), IRESA-University of Sousse, Regional Research Centre on Horticulture and Organic Agriculture, 4042, Chott Mariem, Tunisia.
Fusarium dry rot, Pythium leak and Southern blight diseases caused by various Fusarium species, Pythium aphanidermatum, and Agroathelia rolfsii, respectively, are responsible for important losses of tuber at the post-harvest stage in Tunisia. In the present study, six Bacillus strains, isolated from wild solanaceous plants, were screened for their abilities to inhibit potato pathogens in vitro and on potato tubers. Based on the dual culture assays, the whole cell suspensions of B.
View Article and Find Full Text PDFTowards a 3D-Printed Millifluidic Device for Investigating Cellular Processes.
Micromachines (Basel)
October 2024
Institute for Macromolecular Chemistry, University of Freiburg, 79104 Freiburg, Germany.
Microfluidic devices (µFDs) have been explored extensively in drug screening and studying cellular processes such as migration and metastasis. However, the fabrication and implementation of microfluidic devices pose cost and logistical challenges that limit wider-spread adoption. Despite these challenges, light-based 3D printing offers a potential alternative to device fabrication.
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