Two closely related copper proteins, phenoloxidase and haemocyanin, are known to be involved in different physiological functions such as the primary immune response and oxygen transport. Although the proteins differ structurally, they have the same active site by which dioxygen is bound. Recent results reveal that haemocyanin also exhibits phenoloxidase activity. A scenario is proposed for the evolutionary relationships among copper oxygen-binding proteins (COPs).
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http://dx.doi.org/10.1242/jeb.203.12.1777 | DOI Listing |
J Hazard Mater
September 2024
College of Environment and Resources, Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, Hunan, China. Electronic address:
Laccases are the most commonly used agents for the treatment of phenolic pollutants. To address the instability and high cost of natural laccases, we investigated nucleobase-modulated copper nanomaterial with laccase-like activity. Various nucleobases, including adenine, guanine, cytosine, and thymine, were investigated as templates for Cu reduction and copper nanomaterials formation due to their coordination capacity.
View Article and Find Full Text PDFBMC Vet Res
May 2024
Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, 1 Shields Ave, 2112 Tupper Hall, Davis, CA, 95616, USA.
Background: Hypoxia is a detrimental factor in solid tumors, leading to aggressiveness and therapy resistance. OMX, a tunable oxygen carrier from the heme nitric oxide/oxygen-binding (H-NOX) protein family, has the potential to reduce tumor hypoxia. [F]Fluoromisonidazole ([F]FMISO) positron emission tomography (PET) is the most widely used and investigated method for non-invasive imaging of tumor hypoxia.
View Article and Find Full Text PDFStructure
June 2024
Laboratorio de Bioquímica y Biología Molecular, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago 780003, Chile. Electronic address:
Mollusk hemocyanins, among the largest known proteins, are used as immunostimulants in biomedical and clinical applications. The hemocyanin of the Chilean gastropod Concholepas concholepas (CCH) exhibits unique properties, which makes it safe and effective for human immunotherapy, as observed in animal models of bladder cancer and melanoma, and dendritical cell vaccine trials. Despite its potential, the structure and amino acid sequence of CCH remain unknown.
View Article and Find Full Text PDFProtein Sci
April 2023
Department of Chemical Physiology and Biochemistry, Oregon Health and Science University, Portland, Oregon, USA.
Peptidylglycine monooxygenase (PHM) is essential for the biosynthesis of many neuroendocrine peptides via a copper-dependent hydroxylation of a glycine-extended pro-peptide. The "canonical" mechanism requires the transfer of two electrons from one mononuclear copper (CuH, H-site) to a second mononuclear copper (CuM, M-site) which is the site of oxygen binding and catalysis. In most crystal structures the copper centers are separated by 11 Å of disordered solvent, but recent work has established that a PHM variant H108A forms a closed conformer in the presence of citrate with a reduced Cu-Cu site separation of ~4 Å.
View Article and Find Full Text PDFNanomaterials (Basel)
October 2022
Department of Chemistry, ICES, CSET, University of South Africa, Florida Science Campus, Roodepoort 1710, South Africa.
An alternative electrosynthesis of PdTe, using the electrochemical atomic layer deposition (E-ALD) method, is reported. The cyclic voltammetry technique was used to analyze Au substrate in copper (Cu), and a tellurous (Te) solution was used to identify UPDs and set the E-ALD cycle program. Results obtained using atomic force microscopy (AFM) and scanning electron microscopy (SEM) techniques reveal the nanometer-sized flat morphology of the systems, indicating the epitaxial characteristics of Pd and PdTe nanofilms.
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