The aim of this study was to examine the bacteriostatic and bactericidal effects of the components of RC-Prep: EDTA, urea peroxide and glycol. The minimal inhibitory concentration and the minimal bactericidic concentration of EDTA, urea peroxide and glycol were tested on the viability of Streptococcus sobrinus (S. sobrinus). The combined antibacterial effect of these components was also examined on S. sobrinus. The minimal inhibitory concentration of EDTA was found to be 0.125%, of urea peroxide 0.25%, and of glycol 30%. The minimal bactericidic concentration of EDTA was 0.25%, of urea peroxide 0.5% and of glycol 50%. An antibacterial synergistic effect was found between specific combinations of urea peroxide, EDTA and glycol. No antagonistic effects were found in the various combinations between the components. The antimicrobial effect of the components of RC-Prep was enhanced in specific combinations of the ingredients. This effect might contribute to the intracanal cleansing properties of this medication.
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