Bovine spongiform encephalopathy (BSE) is a prion-associated disease where the infectious agent is thought to be a host-encoded protein with a protease-resistant conformation (PrP(Sc)). Here, data are presented on the solubilization of purified murine BSE material, using guanidine-HCl as a denaturing agent. This treatment led to loss of infectivity, which was partially recovered on renaturation after dialysis to remove the chaotropic agent. The renatured product was then fractionated on an isopycnic sucrose-density gradient and the fractions were analysed for the presence of PrP(Sc), nucleic acids and infectivity. It was found that the major part of PrP(Sc) (>90%) and the endogenous nucleic acids did not contribute towards the formation of infectious particles on renaturation. Infectivity was distributed in the top three, low-density fractions. Among these, the presence of considerable infectivity in the fraction of lowest density, with barely detectable PrP(Sc), is of particular interest.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1099/0022-1317-81-6-1615 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Study and In Vitro Evaluation of the Bioactive Compounds of Broad Bean Sprouts for the Treatment of Parkinson's Syndrome.
Molecules
October 2024
Nanjing Core Tech Biomedical Co., Ltd., Nanjing 211100, China.
Levodopa (LD) is the first discovered and the most promising and effective medication for Parkinson's disease (PD). As the first identified natural source of LD, L. (broad beans), especially its sprouts, has been confirmed to contain many other potential bioactive compounds that could also be therapeutic for PD.
View Article and Find Full Text PDFassessment of neuroinflammatory cytokines in different stages of ovine natural prion disease.
Front Vet Sci
October 2024
Research Centre for Encephalopathies and Transmissible Emerging Diseases. Institute for Health Research Aragón (IIS) - WOAH Reference Laboratory for BSE and Scrapie, University of Zaragoza, Zaragoza, Spain.
Introduction: According to the neuroinflammatory hypothesis, a cytokine-mediated host innate immune response may be involved in the mechanisms that contribute to the process of neurodegeneration. Specifically, regarding prion diseases, some experimental murine models have evidenced an altered profile of inflammatory intermediaries. However, the local inflammatory response has rarely been assessed, and never in tissues from different natural models throughout the progression of neurodegeneration.
View Article and Find Full Text PDFDevelopment of a small molecule-based two-photon photosensitizer for targeting cancer cells.
J Mater Chem B
December 2024
Department of Energy Systems Research and Department of Chemistry, Ajou University, Suwon 16499, Korea.
Article Synopsis
- Researchers developed BSe-B, a new photosensitizer for two-photon photodynamic therapy (TP-PDT) that targets cancer cells while sparing normal cells.
- BSe-B combines a selenium-based dye with a biotin ligand for cancer selectivity, showcasing low toxicity and effective generation of reactive oxygen species.
- The study's findings confirm BSe-B's potential for precise cancer treatment and imaging, making it a valuable tool for both diagnosis and therapy in cancer care.
Codon and Signal Peptide Optimization to Enhance Therapeutic Antibody Production from CHO Cells.
Methods Mol Biol
October 2024
Molecular Biotechnology Laboratory, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
A simple and rapid method for reproducible transient expression experiments to quickly identify the best conditions before the development of a stable CHO cell line is described, using adalimumab as a model antibody. Firstly, the signal peptide (SP) is optimized using different in silico programs, of which those having a D score of more than 0.85 will be selected.
View Article and Find Full Text PDFIncreased bone mass but delayed mineralization: in vivo and in vitro study for zoledronate in bone regeneration.
BMC Oral Health
September 2024
Fujian Key Laboratory of Oral Diseases, School and Hospital of Stomatology, Fujian Medical University, Fujian, China.
Background: Bisphosphonates (BPs) are widely used to inhibit excessive osteoclast activity. However, the potential to compromise bone defect healing has limited their broader application. To better understand the influence of BPs on bone regeneration, we established a bone grafting model with Zoledronate administration, aiming to deepen the understanding of bone remodeling and mineralization processes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!