Croat Med J
Laboratory for Clinical and Forensic Genetics, Split University Hospital and School of Medicine, Spincićeva 1, 21000 Split, Croatia.
Published: March 2000
DNA technology has taken an irreplaceable position in the field of the forensic sciences. Since 1985, when Peter Gill and Alex Jeffreys first applied DNA technology to forensic problems, to the present, more than 50,000 cases worldwide have been solved through the use of DNA based technology. Although the development of DNA typing in forensic science has been extremely rapid, today we are witnessing a new era of DNA technology including automation and miniaturization. In forensic science, DNA analysis has become "the new form of scientific evidence" and has come under public scrutiny and the demand to show competence. More and more courts admit the DNA based evidence. We believe that in the near future this technology will be generally accepted in the legal system. There are two main applications of DNA analysis in forensic medicine: criminal investigation and paternity testing. In this article we present background information on DNA, human genetics, and the application of DNA analysis to legal problems, as well as the commonly applied respective mathematics.
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Cell Physiol Biochem
January 2025
Pomeranian University in Słupsk, Institute of Biology, Arciszewski St. 22 B, PL 76-200 Słupsk, Poland.
Background/aims: DNA isolation is the initial process in genetic research. The product is used in many PCR reactions (PCR-RFLP, Real-Time PCR, multiplex PCR). That is why it is important to optimize DNA isolation protocol to obtain a good quality of DNA.
View Article and Find Full Text PDFChem Asian J
March 2025
National Institute of Technology Rourkela, Chemistry, NIT Campus, 769008, Rourkela, INDIA.
Here, two mixed-ligand mononuclear [Cu(L1)py] (1), [Cu(L2)Him] (2) and one dinuclear copper(II) complex [Cu2(L3)2(DMSO)(MeOH)] (3) were isolated in solid state and characterized through SC-XRD.- Herein, we highlight the solution behavior of these complexes in solution medium through HRMS and ESR. Though the complexes maintain their integrity with respect to the ligand coordination, there is solvent or co-ligand exchange and generation of both [Cu(L)(py/Him)] or [Cu(L)(H2O)] species.
View Article and Find Full Text PDFAnal Chem
March 2025
Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.
5-Methylcytosine (5mC) functions as a well-characterized epigenetic DNA mark in nuclear DNA, but its presence in mitochondrial DNA (mtDNA) remains elusive. Here, we report a new and rigorous nuclear DNA (nDNA)-excluding mass spectrometry assay enabling the reliable and accurate identification of 5mC in mtDNA for the first time. First, circular mtDNA is enriched over 809-946-fold by combining alkaline lysis and linear DNA-specific RecBCD cutting; nDNA accounts for ∼12-19% of the DNA remaining after this step.
View Article and Find Full Text PDFInt J Cosmet Sci
March 2025
Hangzhou Shiguang Xinya Biotechnology Co., Ltd., Hangzhou, China.
Objective: The study investigated effects of peony callus extracts (PCE) on the protective efficacy against Ultraviolet B (UVB)-induced photoageing, using in vitro and in vivo studies. The research focused on PCE's ability to protect against inflammatory factors, DNA damage and accumulation of senescent cells, along with the evaluation of the extract's potential anti-photoageing benefits to skin.
Methods: Human keratinocyte cell line (HaCaT cells), mast cells and fibroblasts were used to evaluate the role of PCE in anti-photoageing.
Biosaf Health
August 2024
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
Synthetic biology is a crucial component of the "cyber-biological revolution" in this new industrial revolution. Owing to breakthroughs in synthetic biology, deoxyribonucleic acid (DNA), the storehouse of hereditary material in biological systems, can now be used as a medium for storage (synthesis) and reading (sequencing) of information. However, integrating synthetic biology with computerization has also caused cyberbiosecurity concerns, encompassing biosecurity and information security issues.
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