The dependence of the sensitivity of a microbial biosensor of anionic surfactants (AS) on the growth phase of Pseudomonas rathonis T, a strain capable of degrading surfactants, was studied. Correlations were found between the optimum values of temperature and pH of microbial growth, substrate utilization, and functional performance of the microbial biosensor. These results allow the process of AS detection to be optimized.
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Pseudomonas strains (n = 8), isolated from soil and attributed to a new species of Pseudomonas rathonis have been studied by the methods of polyphasic taxonomic analysis. The data of 16 S rRNA sequence, sequence of gyr B-gene, DNA-DNA molecular hybridization, data of fatty acid composition and phenotypical analysis proved, that the studied bacteria belonged to "Pseudomonas aeruginosa-complex". The set of characteristics to differentiate Pseudomonas rathonis from other 11 species of this complex has been proposed.
View Article and Find Full Text PDFCharacterization of Pseudomonas species isolated from the rhizosphere of plants grown in serozem soil, semi arid region of Uzbekistan.
ScientificWorldJournal
July 2005
Center of Agroecology, University of Agriculture, Tashkent 700128, Uzbekistan.
Collections of native Pseudomonas spp. are kept at the NCAM of Uzbekistan. Some of those organisms were isolated from the rhizosphere of cotton, wheat, corn, melon, alfalfa, and tomato grown in field locations within a semi-arid region of Uzbekistan.
View Article and Find Full Text PDFPseudomonas strains isolated from soil and rhizosphere and designated as "Pseudomonas rathonis", Pseudomonas sp. B and Pseudomonas sp. C have been characterized by 113 phenotypic properties and studied by methods of molecular-genetic analysis.
View Article and Find Full Text PDFStrain Comamonas testosteroni TI, capable of degrading the nonionic surfactant (NIS) nonylphenolethoxylate (OP-10), was used for constructing a pilot cellular biosensor. The lower NIS detection limit for the biosensor was 0.25 mg/l.
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