The taxonomic composition of denitrifying bacteria in soddy podzolic soil was studied by the succession analysis method. This method revealed a significant variation in the taxonomic composition of denitrifying microorganisms in the course of succession. In contrast to succession analysis, the single microbiological analysis of soil samples reflected only the late stage of succession and thus led to an underestimation of the major members of succession. Myxobacteria were found to be the most active denitrifiers at the early stages of succession, whereas bacilli dominated at its late stages. The bacilli were represented by three facultatively anaerobic species, Bacillus cereus, Bac. circulans, and Bac. polymyxa.
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Biomolecules
January 2025
Xingzhi College, Zhejiang Normal University, Jinhua 321100, China.
Nitrite reductases play a crucial role in the nitrogen cycle, demonstrating significant potential for applications in the food industry and environmental remediation, particularly for nitrite degradation and detection. In this study, we identified a novel nitrite reductase (NiR) from a newly isolated denitrifying bacterium, YD01. We constructed a heterologous expression system using BL21/pET28a-Nir, which exhibited remarkable nitrite reductase enzyme activity of 29 U/mL in the culture broth, substantially higher than that reported for other strains.
View Article and Find Full Text PDFBioresour Technol
January 2025
Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044 China. Electronic address:
Iron-carbon (Fe-C) based biofilters have shown significant advantages in treating mariculture wastewater by facilitating the mixotrophic heterotrophic nitrification-aerobic denitrification (HNAD) process. However, the effects of Fe-C materials and varying carbon-to-nitrogen (C/N) ratios on N removal and C reduction performance remain insufficiently explored. This study demonstrated that the Fe-C biofilter (R-Fe) achieved significantly higher NO-N removal efficiency (65.
View Article and Find Full Text PDFWater Res
January 2025
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, Shandong, China; Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, Shandong, China. Electronic address:
Sulfur-siderite driven autotrophic denitrification (SSAD) has received increasing attention for nutrient removal in constructed wetlands (CWs). Nevertheless, its effectiveness in simultaneous water purification and greenhouse gases (GHGs) reduction remains obscure. In this study, three vertical flow constructed wetlands (VFCWs), filled with quartz sand (CCW), sulfur (S-CW), and sulfur-siderite mixed substrates (SS-CW), were constructed to investigate the underlying mechanisms of SSAD on water purification enhancement and GHGs reduction.
View Article and Find Full Text PDFBioresour Technol
January 2025
Water Science and Technology Group (WaSTe), Department of Civil Engineering, University of Salerno, Via Giovanni Paolo II 132, 84084 Fisciano, SA, Italy. Electronic address:
In this work, the effect of the electro-assisted Fenton (EAF) process on the bacterial community of a moving bed biofilm reactor (MBBR) for olive mill wastewater (OMW) co-treatment with urban wastewater (UWW) was investigated. According to metagenomic analysis, pre-treatment by EAF, while removing total phenols (TPHs) up to 84 % ± 3 % and improving biodegradability of OMW from 0.38 to 0.
View Article and Find Full Text PDFWaste Manag
January 2025
Guangxi Bossco Environmental Protection Technology Co., Ltd., Nanning 530007, China.
Ammonia nitrogen (NH-N) discharge has caused eutrophication of water bodies and harm to humans and organisms. In this work, polyvinyl alcohol (PVA), sodium carboxymethyl cellulose (CMC), diatomite (DE), and FeO were used to prepare magnetic immobilized carriers by encapsulating microorganisms for the treatment of NH-N wastewater. The response surface methodology was used to explore the optimal ratio of the immobilized carriers.
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