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Colorimetric polymerase chain reaction mediated by pH indicator: Rapid detection of drug-resistant nosocomial bacteria.
Talanta
March 2025
Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13120, South Korea. Electronic address:
Polymerase chain reaction (PCR) is the gold standard for molecular diagnoses due to its high sensitivity and accuracy. However, conventional PCR exhibits limitations including extended analysis time and complicated sample treatment. To shorten the analysis time of standard three-step PCR, we employed a two-step PCR allowing for fast amplification of nucleic acid in a chip format.
View Article and Find Full Text PDFA fluorescence based dual sensor for Zn and PO and the application of soft computing methods to predict machine learning outcomes.
Phys Chem Chem Phys
March 2024
Department of Chemistry, Vidyasagar University, Midnapore 721102, West Bengal, India.
A phenolphthalein-based Schiff base, 3,3-bis-{4-hydroxy-3-[(pyridine-2-ylmethylimino)-methyl]-phenyl}-3-isobenzofuran-1-one (PAP), has been synthesized and used for selective fluorescence 'turn on' and 'turn off' sensing of Zn and PO respectively. The limit of detection using the 3 method for Zn is found to be 19.3 nM and that for PO is 8.
View Article and Find Full Text PDFIntraoperative Neurophysiological Monitoring in Corrective Surgery of Scoliosis - Experience at a Tertiary Care Hospital.
J Coll Physicians Surg Pak
March 2024
Department of Spine Surgery, Combined Military Hospital, Rawalpindi, Pakistan.
Objective: To evaluate the role of intraoperative neurophysiological monitoring (IONM) in reducing the postoperative neurologic deficit following corrective surgery of scoliosis.
Study Design: Observational Study. Place and Duration of the Study: Spine Surgery Department, Combined Military Hospital, Rawalpindi, from December 2022 to May 2023.
Visual authenticating hazardous adulterant phenolphthalein in slimming foods: Target-mimicking hapten epitope improved immunoassay.
Food Chem
July 2024
Guangdong Provincial Key Laboratory of Food Quality and Safety/Nation-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou 510642, China. Electronic address:
Screening for the hazardous adulterant phenolphthalein (PTH) in slimming foods is necessary. Herein, the linkage of the PTH target epitope with various spacer arms was proposed for hapten design, aiming to produce highly sensitive and specific antibodies targeting PTH. To understand the influence of spacer arms on epitope, comprehensive evaluations were conducted using computer-aided chemistry and animal immunization.
View Article and Find Full Text PDFEffectiveness of Thymol Blue and Phenolphthalein in Sol-Gel Coatings for Sensing and Inhibiting Corrosion on Mild Steel.
Langmuir
March 2024
Centre for Sol-Gel Coatings, International Advanced Research Centre for Powder Metallurgy and New Materials (ARCI), Balapur, Hyderabad 500 005, Telangana, India.
Development of smart coatings incorporated with corrosion indicators that can warn of corrosion through signaling the alteration of pH or metal-ion concentration at the corrosion site, and simultaneously mitigate further corrosion, stands out as a highly efficient and economical approach to address corrosion issues. In this context, the present work provides a comprehensive comparison on the effectiveness of thymol blue (TB) and phenolphthalein (PhPh) in both sensing and inhibiting corrosion on mild steel. While most of the works primarily focused on independently investigating the corrosion indication behavior of different active agents, our study intends to offer valuable insights through the comparative analysis of TB and PhPh in making well-informed decisions when selecting the most effective indicator for a given set of application scenarios.
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