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Recent Status on Lactate Monitoring in Sweat Using Biosensors: Can This Approach Be an Alternative to Blood Detection?
Biosensors (Basel)
December 2024
Microsis srl, Via degli Olmetti, 8a, 00060 Formello, Italy.
Recent studies have shown that lactate is a molecule that plays an indispensable role in various physiological cellular processes, such as energy metabolism and signal transductions related to immune and inflammatory processes. For these reasons, interest in its detection using biosensors for non-invasive analyses of sweat during sports activity and in clinical reasons assessments has increased. In this minireview, an in-depth study was carried out on biosensors that exploited using electrochemical methods and innovative nanomaterials for lactate detection in sweat.
View Article and Find Full Text PDFFc-binding M13 phage-enhanced electrochemical biosensors for influenza virus detection.
Biosens Bioelectron
January 2025
Department of Integrative Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 16419, Republic of Korea; Center for Biologics, Sungkyunkwan University, Suwon, Gyeonggi-do, 16419, Republic of Korea. Electronic address:
The importance of in vitro diagnostics (IVDs) has significantly increased, driving the demand for rapid and sensitive diagnostic platforms. Molecular probes play a pivotal role in improving the sensitivity and accuracy of IVDs because of their target-specific signal transduction capabilities. Antibodies, which are commonly used as detection probes, face several challenges, including limited stability, high production costs, and low signal output.
View Article and Find Full Text PDFNucleic Acid Aptamer-Based Sensors for Bacteria Detection: A Review.
Bioessays
January 2025
Department of Biology and Medicine ,college of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Bacteria have a significant impact on human production and life, endangering human life and health, so rapid detection of infectious agents is essential to improve human health. Aptamers, which are pieces of oligonucleotides (DNA or RNA) have been applied to biosensors for bacteria detection due to their high affinity, selectivity, robust chemical stability, and their compatibility with various signal amplification and signal transduction mechanisms. In this review, we summarize the different bacterial aptamers selected in recent years using SELEX technology and discuss the differences in optical and electrochemical bacterial aptamer sensors.
View Article and Find Full Text PDFRapid and receptor-free Prussian blue electrochemical sensor for the detection of pathogenic bacteria in blood.
Bioelectrochemistry
January 2025
Department of Mechanical Engineering, Ajou University, South Korea. Electronic address:
Bloodstream bacterial infections, a major health concern due to rising sepsis rates, require prompt, cost-effective diagnostics. Conventional methods, like CO-based transduction, face challenges such as volatile metabolites, delayed gas-phase signaling, and the need for additional instruments, whereas electrochemical sensors provide rapid, sensitive, and efficient real-time detection. In this study, we developed a bioreceptor-free Prussian blue (PB) sensor platform for real-time bacterial growth monitoring in blood culture.
View Article and Find Full Text PDFWhole-cell aptamer-based techniques for rapid bacterial detection: Alternatives to traditional methods.
J Pharm Biomed Anal
March 2025
University Grenoble Alpes, DPM UMR 5063, CNRS, Grenoble F-38041, France. Electronic address:
Article Synopsis
- Bacterial infectious diseases pose a significant public health challenge, especially with rising antibiotic resistance, making fast detection of pathogens essential for control measures.
- Current methods rely on culture techniques, which are time-consuming and require special equipment, leading to a need for alternative detection methods that can quickly identify whole bacteria without culture.
- Aptamer-based assays are emerging as a promising alternative, offering benefits such as stability, specificity, and cost-effectiveness, with various detection techniques like optical and electrochemical methods enhancing their utility in diagnosing bacterial infections.
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