AI Article Synopsis
- A study identified six strains of ice-nucleating bacteria with site-specific endonucleases from a collection of 45 strains found in plant rhizospheres.
- Three endonucleases (Pfl211I, Psp8I, Psp23I) were isolated from two types of Pseudomonas bacteria, including Pseudomonas fluorescens.
- Endonucleases Pfl21I and Psp23I target the DNA sequence 5'-CTGCA decrease G-3', while Psp8I targets 5'-G decrease GATCC-3', and exhibit similarities to established endonucleases PstI and BamHI.
Article Abstract
Six strains containing site-specific endonucleases II were selected from a collection of 45 ice-nucleating bacterial strains isolated from rhizosphere of plants growing in various geographical regions. Endonucleases Pfl211I, Psp8I, and Psp23I were isolated and purified from two Pseudomonas sp. strains and a Pseudomonas fluorescens strain. Restriction endonucleases Pfl21I and Psp23I were shown to recognize and cleave the DNA nucleotide sequence 5'-CTGCA decrease G-3'. Endonuclease Psp8I recognized and cleaved the DNA nucleotide sequence 5'-G decrease GATCC-3'. These endonucleases were found to be true isoschizomers of PstI and BamHI, respectively.
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