AI Article Synopsis
- PDGF and its alpha-receptor were detected at neuromuscular junctions (NMJs) in both humans and mice using specific antibodies.
- The study employed double fluorescence labeling to show that PDGF and its receptor are closely associated with acetylcholine receptors marked by alpha-bungarotoxin.
- This research is the first to provide evidence of the concentration of PDGF and its receptor at NMJs, suggesting a role in communication between nerve and muscle cells.
Article Abstract
Platelet-derived growth factor (PDGF) and its alpha-receptor were localized at human and mouse neuromuscular junctions (NMJs) using specific polyclonal antibodies against each, anti-PDGF-A and anti-PDGF alpha-receptor, respectively. By applying double fluorescence labeling, immunoreactivity for PDGF and its receptor was closely co-localized with acetylcholine receptors, which were identified with alpha-bungarotoxin. PDGF might be involved in the interaction between the presynaptic and postsynaptic components. This is the first demonstration of PDGF and its receptor concentrated at human and mouse NMJs.
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| http://dx.doi.org/10.1620/tjem.189.239 | DOI Listing |
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