To elucidate the molecular basis underlying quinolinate metabolism, the cDNA encoding ACMSDase was cloned.
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Identification and functional analyses of drought stress resistance genes by transcriptomics of the Mongolian grassland plant Chloris virgata.
BMC Plant Biol
January 2025
Laboratory of Plant Chemical Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
Background: Mongolian grasslands, including the Gobi Desert, have been exposed to drought conditions with few rains. In such harsh environments, plants with highly resistant abilities against drought stress survive over long periods. We hypothesized that these plants could harbor novel and valuable genes for enhancing drought stress resistance.
View Article and Find Full Text PDFInsights into the human cDNA: A descriptive study using library screening in yeast.
J Genet Eng Biotechnol
December 2024
Center for Genomics, Helmy Institute for Medical Sciences, Zewail City of Science and Technology, Giza, Egypt; University of Science and Technology, Zewail City of Science and Technology, Giza, Egypt. Electronic address:
The utilization of human cDNA libraries in yeast genetic screens is an approach that has been used to identify novel gene functions and/or genetic and physical interaction partners through forward genetics using yeast two-hybrid (Y2H) and classical cDNA library screens. Here, we summarize several challenges that have been observed during the implementation of human cDNA library screens in Saccharomyces cerevisiae (budding yeast). Upon the utilization of DNA repair deficient-yeast strains to identify novel genes that rescue the toxic effect of DNA-damage inducing drugs, we have observed a wide range of transcripts that could rescue the strains.
View Article and Find Full Text PDFIdentification and pharmacological characterization of pH-sensitive chloride channels in the fall armyworm, Spodoptera frugiperda.
Insect Biochem Mol Biol
December 2024
The Key Laboratory of Agri-products Quality and Biosafety, Ministry of Education, Anhui Province Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, PR China; Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, PR China. Electronic address:
Article Synopsis
- pH-sensitive chloride channels (pHCls) are found only in invertebrates and are vital for processes like fluid regulation and food intake, with this study focusing on two specific subunits, SfpHCl1 and SfpHCl2, from the fall armyworm.
- Both subunits function similarly when expressed in frog oocytes, showing chloride selectivity with their activity impacted by changes in extracellular pH and activated by zinc ions and the insecticide emamectin benzoate.
- The study reveals significant differences in activation and deactivation properties between SfpHCl1 and SfpHCl2, highlighting their potential as targets for developing specialized insecticides.
Comparative Characterization of Three Homologous Glutathione Transferases from the Weed .
Foods
November 2024
Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855 Athens, Greece.
The comparative analysis of homologous enzymes is a valuable approach for elucidating enzymes' structure-function relationships. Glutathione transferases (GSTs, EC. 2.
View Article and Find Full Text PDFGenome-Wide Characterization and Expression Profiling of Phytosulfokine Receptor Genes (s) in with Docking Simulations of Their Interactions with Phytosulfokine (PSK): A Bioinformatics Study.
Genes (Basel)
October 2024
Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia.
The phytosulfokine receptor () gene family plays a crucial role in regulating plant growth, development, and stress response. Here, the gene family was characterized in L. The study aimed to bridge knowledge gaps and clarify the functional roles of to create a solid foundation for examining the structure, functions, and regulatory aspects.
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