Pectate lyase A is secreted by Erwinia chrysanthemi and is a virulence factor for soft rot diseases in plants. Crystals of pectate lyase A were obtained by vapor-diffusion techniques in the presence of polyethylene glycol. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 48.96, b = 148.86, c = 78.61 A, beta = 97.32 degrees. The crystals contain two protein molecules of 38 kDa per asymmetric unit and diffract to 2.4 A using Cu Kalpha radiation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1107/s0907444999016807 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Clarification of Bio-Degumming Enzymes Based on a Visual Analysis of the Hemp Roving Structure.
Polymers (Basel)
December 2024
Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Hemp fibers, recognized for their breathability, specific strength, and ultraviolet resistance, are widely utilized in textile manufacturing and composite materials. Bio-degumming is a promising alternative technology to traditional chemical degumming that can be used to produce hemp fibers due to its eco-friendly nature. However, its lower efficiency has hindered its widespread adoption.
View Article and Find Full Text PDFSelection of alkaliphilic Bacillus pectate lyases based on reactivity and pH-dependent stability in simulated environment for industrial applications.
Carbohydr Res
December 2024
Quantitative Biology Lab, Department of Integrative Biology, School of Bio Sciences and Technology, Vellore Institute of Technology (VIT Deemed to Be University), Vellore, Tamil Nadu, India. Electronic address:
Pectate lyases, known for their alkaliphilic nature, are ideal for industrial applications that require specific pH conditions, particularly in industries such as textiles and pulp extraction. These enzymes, primarily from the polysaccharide lyase family 1 (PL1) of different microbial sources, play a vital role in polysaccharide degradation. Given the potent pectinolytic activity of Bacillus pectate lyases, targeting these enzymes is crucial for identifying the most effective candidates.
View Article and Find Full Text PDFComparative transcriptome analysis in two contrasting genotypes for Sclerotinia sclerotiorum resistance in sunflower.
PLoS One
December 2024
Liaoning Academy of Agricultural Sciences, Shenyang, China.
Sclerotinia sclerotiorum as a necrotrophic fungus causes the devastating diseases in many important oilseed crops worldwide. The preferred strategy for controlling S. sclerotiorum is to develop resistant varieties, but the molecular mechanisms underlying S.
View Article and Find Full Text PDFA PL1 family pectate lyase gene was the pathogenic factor of 5T-1 causing potato common scab.
Front Microbiol
November 2024
College of Plant Protection, Gansu Agricultural University, Lanzhou, China.
Pectate lyases (PL), as important polysaccharide lyases, play an important role in the infection of host plants by pathogenic. A previous study found that the PL gene was up-regulated in the interaction between 5T-1 and potatoes. In this study, 5T-1 was used as the study object, and its gene function was investigated using bioinformatics analysis, prokaryotic expression, and CRISPR-Cas9 technology.
View Article and Find Full Text PDFMdMYB54 reduces disease severity caused by Fusarium solani in apple by modulating cell wall cellulose and pectate lyase-dependent defense.
Plant J
December 2024
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, China.
The plant cell wall is the first barrier against pathogen invasion. Fusarium solani is the primary pathogen responsible for apple replant disease. In this study, we identified an MYB protein, MdMYB54, which interacts with the positive regulator of F.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!