The morphology of Trichoderma reesei Rut C-30, during submerged cultivations in shake flask, was examined. The influence of the size inoculum and the composition of the fermentation medium on the morphology and cellulase production was studied. Different inoculum sizes were studied but the significative change in fungus morphology was observed for spores concentration between 10(5) and 10(7) spores/ml (i.e. 10(2) and 10(4) spores/ml in pre-culture medium). In the medium without Tween 80, at low inoculum size, the majority of the pellets were large and well individualized, in contrast, at higher inoculation densities small flocs were obtained, with higher production of soluble protein and higher filter paper activity. It was found that the average pellet size seems to be inversely proportional to the inoculum size. Medium composition, namely Tween 80, also influences the morphology of T. reesei Rut C-30 and enzyme production. The presence of Tween 80 in fermentation medium inhibited the pellet formation of this strain.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0141-0229(99)00166-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mechanism of improved cellulase production by Trichoderma reesei in water-supply solid-state fermentation.
Bioresour Technol
December 2024
Sanya Institute of Nanjing Agricultural University, Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:
High production cost of cellulases limits its commercial application on lignocellulose. Solid-state fermentation (SSF) has special advantages of water and energy conservation, however, the lack of free water and water loss during fermentation limits its application. In this paper, a constructed water-supply SSF was used to improve carboxymethyl cellulose activity and filter paper activity of 1.
View Article and Find Full Text PDFGene Expression by a Model Fungus in the Ascomycota Provides Insight Into the Decay of Fungal Necromass.
Environ Microbiol
December 2024
Department of Plant and Microbial Biology, College of Biological Sciences, University of Minnesota, Twin Cities, Minnesota, USA.
Article Synopsis
- Dead fungal cells, referred to as necromass, contribute significantly to long-term soil carbon pools, but the genes responsible for their decomposition are not well understood.
- * The study focused on the fungus Trichoderma reesei and its response to low and high melanin levels in the necromass of another fungus, Hyaloscypha bicolor, revealing over 100 up-regulated genes for carbohydrate-active enzymes (CAZymes) in the presence of necromass compared to glucose.
- * Differential expression of specific genes related to proteases and laccases was noted, particularly linked to the breakdown of melanin, offering insights into the factors affecting carbon turnover rates in this underexplored area of soil biology.
Exploring the Synergistic Secretome: Insights from Co-Cultivation of and RUT-C30.
J Fungi (Basel)
September 2024
Laboratory of Enzymology, Department of Cell Biology, University of Brasília, Brasília 70910-900, DF, Brazil.
The spectrum of enzymes required for complete lignocellulosic waste hydrolysis is too diverse to be secreted by a single organism. An alternative is to employ fungal co-cultures to obtain more diverse and complete enzymatic cocktails without the need to mix enzymes during downstream processing. This study evaluated the co-cultivation of and RUT-C30 in different conditions using sugarcane bagasse as the carbon source.
View Article and Find Full Text PDFEnhanced glucose-1-phosphate production from corn stover using cellulases with reduced β-glucosidase activity via Trbgl1 gene knockout in Trichoderma reesei Rut C30.
Enzyme Microb Technol
October 2024
School of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, China. Electronic address:
The scarcity of cellulases with low β-glucosidase activity poses a significant technological challenge in precisely controlling the partial hydrolysis of lignocellulose to cellobiose, crucial for producing high-value chemicals such as starch, inositol, and NMN. Trichoderma reesei is a primary strain in cellulase production. Therefore, this study targeted the critical β-glucosidase gene, Trbgl1, resulting in over an 86 % reduction in β-glucosidase activity.
View Article and Find Full Text PDFUpgraded cellulose and xylan digestions for synergistic enhancements of biomass enzymatic saccharification and bioethanol conversion using engineered Trichoderma reesei strains overproducing mushroom LeGH7 enzyme.
Int J Biol Macromol
October 2024
Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation & Molecular Pharmaceutics, Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei Key Laboratory of Industrial Microbiology, School of Life & Health Sciences, Hubei University of Technology, Wuhan, 430068, China; College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
Crop straws provide enormous lignocellulose resources transformable for sustainable biofuels and valuable bioproducts. However, lignocellulose recalcitrance basically restricts essential biomass enzymatic saccharification at large scale. In this study, the mushroom-derived cellobiohydrolase (LeGH7) was introduced into Trichoderma reesei (Rut-C30) to generate two desirable strains, namely GH7-5 and GH7-6.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!