AI Article Synopsis
- Researchers developed a method to study human renal tubular cells by isolating primary cultured proximal tubule (PT) cells from urine, helping to better understand how these cells function.
- They identified PT cells using histochemical techniques and measured Na+/H+ exchanger (NHE) activity by observing intracellular pH changes in response to acid loading.
- The findings indicate that these cultured PT cells show amiloride-resistant NHE-3 activity, highlighting their relevance for studying renal transport functions in a controlled lab setting.
Article Abstract
Aim: To facilitate the understanding of the transporter function of human renal tubular cells, we have developed a simple method using primary cultured proximal tubule (PT) cells isolated from voided urine.
Methods: PT cells grown to confluence on glass coverslips could be identified by parallel arrays of spindle cells and hemicyst formation. Brush-border gamma-glutamyl transpeptidase (gammaGTP) activity was histochemically identified. Apical membrane Na+/H+ exchanger (NHE) activity was measured by monitoring changes in intracellular pH (pHi) after an acid load in a single cell level using the pH-sensitive dye 2'7'-bis-(2-carboxyethyl)-5.6'carboxyfluorescein (BCECF).
Results: Amiloride and 5-(N-ethyl-N-isopropyl) amiloride (EIPA) inhibited the NHE activity with half-maximal inhibition values (IC50) of 15.3 and 4.0 microM, respectively. NHE-3 mRNA was detected by the RT-PCR technique in clonally proliferated PT cells.
Conclusion: These results suggest that cultured PT cells isolated from human urine express amiloride-resistant NHE-3 activity on the apical membranes, which can be compared to functional properties of PT in vivo. Our experimental strategy offers a useful experimental approach to investigating human renal tubular transport function in vitro.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Homeostats: The hidden rulers of ion homeostasis in plants.
Quant Plant Biol
September 2024
Instituto de Ciencias Biológicas, Universidad de Talca, Talca, Chile.
Ion homeostasis is a crucial process in plants that is closely linked to the efficiency of nutrient uptake, stress tolerance and overall plant growth and development. Nevertheless, our understanding of the fundamental processes of ion homeostasis is still incomplete and highly fragmented. Especially at the mechanistic level, we are still in the process of dissecting physiological systems to analyse the different parts in isolation.
View Article and Find Full Text PDFGeneration of effective and specific human TCRs against tumor/testis antigen NY-ESO-1 in mice with humanized T cell recognition system.
Front Immunol
December 2024
Molecular Immunology and Gene Therapy, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Generation of high avidity T cell receptors (TCRs) reactive to tumor-associated antigens (TAA) is impaired by tolerance mechanisms, which is an obstacle to effective T cell therapies for cancer treatment. NY-ESO-1, a human cancer-testis antigen, represents an attractive target for such therapies due to its broad expression in different cancer types and the restricted expression in normal tissues. Utilizing transgenic mice with a diverse human TCR repertoire, we isolated effective TCRs against NY-ESO-1 restricted to HLA-A*02:01.
View Article and Find Full Text PDFMetastatic urothelial carcinoma in multiple appendicular muscles of a cat.
JFMS Open Rep
January 2025
Department of Anatomy, Comparative Pathology and Toxicology, University of Córdoba, Campus de Rabanales, Córdoba, Spain.
Case Summary: A 13-year-old male castrated domestic shorthair cat presented with a 2-month history of progressive lameness, poor appetite and constipation. Physical examination revealed palpable lesions in muscles of several extremities. Ultrasound examination confirmed the presence of round lesions with a hypo- or anechoic centre within the muscles.
View Article and Find Full Text PDFSialoadhesin-dependent susceptibility and replication of porcine reproductive and respiratory syndrome viruses in CD163-expressing cells.
Front Vet Sci
December 2024
Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbuk-do, Republic of Korea.
Understanding the molecular interactions between porcine reproductive and respiratory syndrome viruses (PRRSVs) and host cells is crucial for developing effective strategies against PRRSV. CD163, predominantly expressed in porcine macrophages and monocytes, is a key receptor for PRRSV infection. CD169, also known as Sialoadhesin, has emerged as a potential receptor facilitating PRRSV internalization.
View Article and Find Full Text PDFcapsular polysaccharide A ameliorates ulcerative colitis in rat by recovering intestinal barrier integrity and restoring gut microbiota.
Front Pharmacol
December 2024
Guangzhou ZhiYi Biotechnology Co. Ltd., Guangzhou, China.
() is a Gram-negative, obligate anaerobic, commensal bacterium residing in the human gut and holds therapeutic potential for ulcerative colitis (UC). Previous studies have indicated that capsular polysaccharide A (PSA) of is a crucial component for its effectiveness, possessing various biological activities such as anti-inflammatory, anti-tumor, and immune-modulating effects. We previously isolated and characterized the strain ZY-312 from the feces of a healthy breastfed infant, and extracted its PSA, named TP2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!