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ITS-2 sequences-based identification of Trichogramma species in South America.
Braz J Biol
November 2015
Department of Entomology, University of California, Riverside, CA, USA.
ITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T.
View Article and Find Full Text PDFMolecular identification of nine commercial flaffish species by polymerase chain reaction-restriction fragment length polymorphism analysis of a segment of the cytochrome b region.
J Food Prot
June 2002
Xenética Evolutiva Molecular, Facultade de Ciencias-Bioloxía, Universidade de Vigo, Spain.
Commercial refrigerated or frozen flatfish fillets are sometimes mislabeled, and identification of these mislabeled products is necessary to prevent fraudulent substitution. Identification of nine commercial flatfish species (order Pleuronectiformes), Hippoglossus hippoglossus (halibut), Lepidorhombus boscii (four-spotted scaldfish), Lepidorhombus whiffiagonis (megrin), Platichthys flesus (flounder), Pleuronectes platessa (European plaice), Reinhardtius hippoglossoides (Greenland halibut), Scophthalmus maximus (turbot), Scophthalmus rhombus (brill), and Solea vulgaris (=Solea solea) (sole), was carried out on the basis of the amplification of a 486-bp segment of the mitochondrial genome (tRNA(Glu)/cytochrome b) by using the polymerase chain reaction (PCR) and universal primers. Sequences of PCR-amplified DNA from the flatfish species were used to select eight restriction enzymes (REs).
View Article and Find Full Text PDFIdentification of a MaeI RFLP in the insulin-like growth factor-1 (IGF1) gene of swamp buffaloes (Bubalus b. bubalis kerebau).
Anim Genet
February 2000
Institute of Veterinary Medicine, University of Göttingen, Germany.
Identification of a novel mutation of the CPO gene in a Japanese hereditary coproporphyria family.
Am J Med Genet
November 1998
The Third Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Hereditary coproporphyria (HCP) is an autosomal dominant disease characterized by a deficiency of coproporphyrinogen oxidase (CPO) caused by a mutation in the CPO gene. Only 11 mutations of the gene have been reported in HCP patients. We report another mutation in a Japanese family.
View Article and Find Full Text PDFIdentification and haplotype analysis of apolipoprotein B-100 Arg3500-->Trp mutation in hyperlipidemic Chinese.
Clin Chem
August 1998
Department of Internal Medicine, Wei Gong Memorial Hospital, Miaoli, Taiwan, Republic of China.
DNA screening for apolipoprotein (apo) B-100 mutations was performed in hyperlipidemic Chinese. The apo B-100 gene segment surrounding previously identified familial defective apo B-100 (FDB) mutations was amplified by PCR and subjected to single-strand conformation polymorphism (SSCP) analysis. One subject's aberrant SSCP band was cloned and sequenced to study the molecular lesions.
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