We have constructed a two-dimensional database of the proteome of Haemophilus influenzae, a bacterium of medical interest of which the complete genome, comprising about 1742 open reading frames, has been sequenced. The soluble protein fraction of the microorganism was analyzed by two-dimensional electrophoresis, using immobilized pH gradient strips of various pH regions, gels with different acrylamide concentrations and buffers with different trailing ions. In order to visualize low-copy-number gene products, we employed a series of protein extraction and sample application approaches and several chromatographic steps, including heparin chromatography, chromatofocusing and hydrophobic interaction chromatography. We have also analyzed the cell envelope-bound protein fraction using either immobilized pH gradient strips or a two-detergent system with a cationic detergent in the first and an anionic detergent in the second-dimensional separation. Different proteins (502) were identified by matrix-assisted laser desorption/ionization mass spectrometry and amino acid composition analysis. This is at present one of the largest two-dimensional proteome databases.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/(SICI)1522-2683(20000101)21:2<411::AID-ELPS411>3.0.CO;2-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Potential role of the antimicrobial peptide Tachyplesin III in regulating nontypeable Haemophilus influenzae-induced inflammation in airway epithelial cells.
Arch Microbiol
November 2024
Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
Article Synopsis
- Nontypeable Haemophilus influenzae (NTHi) is a major contributor to inflammation in chronic obstructive pulmonary disease (COPD), necessitating new treatments due to limitations of current drugs.
- A study investigated Tachyplesin III, an antimicrobial peptide from horseshoe crabs, and found it significantly reduced key inflammatory markers (like IL-1β and TNF-α) in human lung cells exposed to NTHi without being toxic.
- The research also showed that Tachyplesin III inhibits the NF-κB/NLRP3 signaling pathway, suggesting it could be a valuable new option for addressing NTHi-related airway inflammation in COPD patients.
Global awareness of antigenic peptides - Unraveling the impact on sickle cell anemia patients in the presence of pathogens: A narrative review.
Medicine (Baltimore)
September 2024
Department of Medical Laboratory Science, Kampala International University, Uganda.
Sickle cell anemia (SCA) is a genetic blood disorder characterized by the production of abnormal hemoglobin S (HbS), leading to sickle-shaped red blood cells and various complications, including increased susceptibility to infections. The presence of antigenic peptides, short amino acid sequences derived from pathogens or altered self-proteins, plays a crucial role in immune responses. This review explores the global awareness of antigenic peptides, their role in immune responses in SCA patients, and the challenges and opportunities in managing infections within this vulnerable population.
View Article and Find Full Text PDFSalivary polyreactive antibodies and are associated with respiratory infection severity in young children with recurrent respiratory infections.
Eur Respir J
October 2024
Department of Pediatric Infectious Diseases and Immunology, Wilhelmina Children's Hospital, Utrecht, The Netherlands
Background: Recurrent respiratory tract infections (rRTIs) are a common reason for immunodiagnostic testing in children, which relies on serum antibody level measurements. However, because RTIs predominantly affect the respiratory mucosa, serum antibodies may inaccurately reflect local immune defences. We investigated antibody responses in saliva and their interplay with the respiratory microbiota in relation to RTI severity and burden in young children with rRTIs.
View Article and Find Full Text PDFEndotypes of severe neutrophilic and eosinophilic asthma from multi-omics integration of U-BIOPRED sputum samples.
Clin Transl Med
July 2024
National Heart and Lung Institute, Imperial College London, London, UK.
Background: Clustering approaches using single omics platforms are increasingly used to characterise molecular phenotypes of eosinophilic and neutrophilic asthma. Effective integration of multi-omics platforms should lead towards greater refinement of asthma endotypes across molecular dimensions and indicate key targets for intervention or biomarker development.
Objectives: To determine whether multi-omics integration of sputum leads to improved granularity of the molecular classification of severe asthma.
Metabolic and Microbial Dysregulation in Preterm Infants with Neonatal Respiratory Distress Syndrome: An Early Developmental Perspective.
J Proteome Res
August 2024
Department of Respiratory and Critical Care Medicine, Peking University People's Hospital, Beijing 100044, China.
Neonatal respiratory distress syndrome (NRDS) is one of the most severe respiratory disorders in preterm infants (PTIs) due to immature lung development. To delineate the serum metabolic alterations and gut microbiota variations in NRDS and assess their implications on neonatal development, we enrolled 13 NRDS neonates and 12 PTIs and collected fecal and serum specimens after birth. Longitudinal fecal sampling was conducted weekly for a month in NRDS neonates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!