AI Article Synopsis
- The study involved photografting glycidylmethacrylate (GMA) onto polystyrene microtitre plates using methanol or butanol with benzophenone as the photoinitiator.
- Alternatively, polyglycidylmethacrylate (PGMA) prepolymers were synthesized and dip-coated onto the plates for improved binding capacity.
- The effectiveness of these methods was tested using peroxidase and led to the development of a myelin basic protein (MBP)-based ELISA for immobilization on epoxy-modified plates.
Article Abstract
Photografting of glycidylmethacrylate (GMA) onto commercially available polystyrene-microtitre plates was carried out in methanol as well as butanol with benzophenone (BP) as photoinitiator. Alternatively, prepolymers of polyglycidylmethacrylate (PGMA) were synthesized in methanol with azo-iso-butyrodinitrile (AIBN) as initiator and dip-coated onto polystyrene-microtitre plates. Both modification methods were tested in order to reach a high binding capacity for proteins. Peroxidase was used as model protein. In addition, the immobilization of myelin basic protein (MBP) to epoxy-modified microtitre plates is shown and a MBP-based ELISA has been developed.
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| http://dx.doi.org/10.1016/s0142-9612(99)00098-8 | DOI Listing |
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