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Shiga toxin-producing Escherichia coli (STEC) is one of the major pathogens responsible for severe foodborne infections, and the common serotypes include E. coli O157, O26, O45, O103, O111, O121, and O145. Vaccination has the potential to prevent STEC infections, but no licensed vaccines are available to provide protection against multiple STEC infections.

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Enteropathogenic (EPEC), necrotoxigenic (NTEC), and Shiga-toxin producing (STEC) are pathotypes responsible for severe clinical forms in humans and animals. They can be shed in the feces of animals with consequent environmental contamination. This study evaluated the antibacterial activity of essential oils (EOs) from oregano (, savory , thyme (, and their blend against EPEC, NTEC, and STEC strains previously isolated from avian fecal samples.

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This investigation aimed to examine the virulence genes and antimicrobial resistance profiles of Shiga toxin-producing (STEC) strains found in diarrheal calves in Xinjiang between 2016 and 2022. A total of 800 samples, including 232 fecal and 568 rectal swabs from calves under 2 months old with diarrhea, were analyzed for Shiga toxin (Stx)-encoding genes using polymerase chain reaction. The study characterized the isolates based on their subtypes, virulence genes, O serogroups, phylogenetic groups, hemolytic phenotypes, antibiotic resistance, and resistance genes.

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Background: Globally, diarrhoeagenic Escherichia coli (DEC) has been implicated in the spread of waterborne diseases and abattoir wastewater has played a role in its dissemination into watersheds. This study isolated and characterised DEC from the abattoir wastewater-impacted Iyi-Etu River and other water sources at the Amansea livestock market settlement.

Methods: A total of 96 water samples comprising river water (upstream, downstream 1, downstream 2), borehole, well, sachet and abattoir wastewater samples were tested for DEC.

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Comparative analyses of persistence traits in O157:H7 strains belonging to different clades including REPEXH01 and REPEXH02 strains.

Front Microbiol

December 2024

Meat Safety and Quality Research Unit, U.S. Department of Agriculture, U.S. Meat Animal Research Center, Clay Center, NE, United States.

Recent application of whole genome sequencing in the investigation of foodborne illness outbreaks has facilitated the identification of Reoccurring, Emerging, or Persistent (REP) bacterial strains that have caused illnesses over extended periods of time. Here, the complete genomes of two O157:H7 (EcO157) outbreak strains belonging to REPEXH01 and REPEXH02, respectively, were sequenced and annotated. Comparative genomics and phenotypic analyses were carried out to identify REP-associated traits.

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