Crystals of the Msx-1 homeodomain-DNA complex have been obtained by hanging-drop vapor diffusion at 293 K in 12% PEG 4000 and 0.1 M sodium acetate pH 4.6. The homeodomain consists of 60 amino acids and is the DNA-binding domain. The DNA in the complex was 16 base pairs with the sequence 5'-TGTCACTAATTGAAGG-3', containing an overhang T at each end. The crystals diffract to 2.15 A (99.8% completeness) using cryogenic (123 K) conditions. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 33.66, b = 60.96, c = 83.37 A. The structure will illuminate the details of Msx-1-DNA binding specificity and clarify its role in transcriptional regulation. Mutations in Msx-1 cause craniofacial deformities in mice.
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Role of DNA conformation & energetic insights in Msx-1-DNA recognition as revealed by molecular dynamics studies on specific and nonspecific complexes.
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October 2001
Michigan State University Chemistry Department, East Lansing Michigan 48824, USA.
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Acta Crystallogr D Biol Crystallogr
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Michigan State University Chemistry Department, East Lansing, MI 48824, USA.
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February 1995
Center for Advanced Biotechnology and Medicine, UMDNJ-Robert Wood Johnson Medical School, Piscataway.
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