The effects of various carbon and nitrogen sources on the synthesis of exopolysaccharides by Rhodotorula acheniorum MC were studied. The dynamic viscosity of cell-free culture broths during exopolysaccharide synthesis were measured. The highest values for the viscosity (10.14 MPa.s) and crude polysaccharide productivity (6.6 g/L) were obtained in a medium supplied with 5% sucrose. Ammonium sulfate was the most favorable nitrogen source for exopolysaccharide synthesis. The value of pH played a determinant role, and the obligatory condition for exopolysaccharide production was low (pH 1.7-2.0) during the fermentation. The chemical composition and sugar constituents of the crude exopolysaccharides were determined. Mannose was the main monosaccharide component, and its concentration was the highest (69.13%) in the crude exopolysaccharide synthesized in the medium that included 5% sucrose as a carbon source.
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http://dx.doi.org/10.1385/abab:81:3:181 | DOI Listing |
Int J Cosmet Sci
October 2007
University of Food Technologies, Department of Organic Chemistry, 4002 Plovdiv, Bulgaria.
Subject of study was the colloid chemical properties of the biopolymers mannan, synthesized from strain Rhodotorula acheniorum MC, and glucomannan, synthesized from strain Sporobolomyces salmonicolor AL(1). Their emulsifying capacity was studied in model systems of aqueous solutions in concentrations from 0.5% to 2.
View Article and Find Full Text PDFFEMS Yeast Res
May 2008
Centro de Recursos Microbiológicos (CREM), Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
Among many isolates that resulted from four independent surveys of yeasts associated with plants in Brazil, the USA, Portugal and Taiwan, we have characterized eighteen basidiomycetous strains, two of which were conspecific with the type strain of Rhodotorula acheniorum, whereas the remaining sixteen isolates appeared not to correspond to any previously described species. Microsatellite-PCR fingerprinting with primers M13 and (GTG)5 confirmed that the latter strains formed three genetically distinct groups. Each group was considered to represent a distinct species based on nucleotide sequences of the D1/D2 domains of the 26S rRNA gene and the internal transcribed spacer (ITS) region.
View Article and Find Full Text PDFAppl Biochem Biotechnol
September 1999
Laboratory of Applied Microbiology, Bulgarian Academy of Sciences, Plovdiv, Bulgaria.
The effects of various carbon and nitrogen sources on the synthesis of exopolysaccharides by Rhodotorula acheniorum MC were studied. The dynamic viscosity of cell-free culture broths during exopolysaccharide synthesis were measured. The highest values for the viscosity (10.
View Article and Find Full Text PDFArch Microbiol
September 1995
UFR de Microbiologie Industrielle et Génétique des Microorganismes, ENSA.M-INRA, Place Viala, F-34060 Montpellier Cédex, France.
Lipid content and composition of fatty acids with 6-25 carbon atoms were studied on strains of the 13 pink or red yeast species belonging to the genus Rhodotorula. The total amount of lipid represented an average of 13% of the dry weight. The neutral and polar lipid fractions were analyzed separately.
View Article and Find Full Text PDFThe descriptions of two new species of the genus Sterigmatomyces, St. nectairii and St. penicillatus are given.
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