SIAH-1 inhibits cell growth by altering the mitotic process.

Oncogene

Laboratoire de Pharmacologie Expérimentale, Hôpital Saint-Louis, Paris, France.

Published: November 1999

SIAH-1, the human homologue of the drosophila seven in absentia gene, is a p53-p21Waf-1 inducible gene. We report that stable transfection with SIAH-1 of the epithelial breast cancer cell line MCF-7 blocks its growth process. The transfectants show a redistribution of SIAH-1 protein within the nucleus, more specifically to the nuclear matrix, associated to dramatic changes in cell morphology and defective mitosis. Multinucleated giant cells (2-12 nuclei in more than 50% cells) were a most striking observation associated with tubulin spindle disorganization and defective cytokinesis. There were also present at high frequency abortive mitotic figures, DNA bridges and persistance of intercellular bridges and midbodies, along with an increased expression of p21Waf-1. These results indicate that the mechanism of growth arrest induced by SIAH-1 in MCF-7 cells involves disorganization of the mitotic program, mainly during nuclei separation and cytokinesis.

Download full-text PDF

Source
http://dx.doi.org/10.1038/sj.onc.1203187DOI Listing

Publication Analysis

Top Keywords

siah-1
5
siah-1 inhibits
4
inhibits cell
4
cell growth
4
growth altering
4
altering mitotic
4
mitotic process
4
process siah-1
4
siah-1 human
4
human homologue
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!