The authors present an account on cultivation findings assembled in defects of the lower extremities of diabetic patients hospitalized at the surgical department of the Pelhrimov hospital. In the polymicrobial flora a relatively high ratio of anaerobic microorganisms was found (58%). Based on the sensitivity, a combination of antibiotics was recommended which could be used in empirical treatment before cultivation results (clindamycin, ofloxacin, gentamycin and azlocillin). The authors prefer results of actual sensitivity tests. The authors discuss principles of collection and processing of microbiological material and treatment. Anaerobic cultivation is specially emphasized.
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