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Simultaneous engineering of natural killer cells for CAR transgenesis and CRISPR-Cas9 knockout using retroviral particles.
Mol Ther Methods Clin Dev
June 2023
Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Natural killer (NK) cells are potent cytotoxic innate lymphocytes that can be used for cancer immunotherapy. Since the balance of signals from activating and inhibitory receptors determines the activity of NK cells, their anti-tumor activity can be potentiated by overexpressing activating receptors or knocking out inhibitory receptors via genome engineering, such as chimeric antigen receptor (CAR) transgenesis and CRISPR-Cas9-mediated gene editing, respectively. Here, we report the development of a one-step strategy for CRISPR-Cas9-mediated gene knockout and CAR transgenesis in NK cells using retroviral particles.
View Article and Find Full Text PDFImproved DNA Vaccine Delivery with Needle-Free Injection Systems.
Vaccines (Basel)
January 2023
PharmaJet, 400 Corporate Circle, Unit N, Golden, CO 80401, USA.
DNA vaccines have inherent advantages compared to other vaccine types, including safety, rapid design and construction, ease and speed to manufacture, and thermostability. However, a major drawback of candidate DNA vaccines delivered by needle and syringe is the poor immunogenicity associated with inefficient cellular uptake of the DNA. This uptake is essential because the target vaccine antigen is produced within cells and then presented to the immune system.
View Article and Find Full Text PDFExpression of the Sweet Potato MYB Transcription Factor Confers Salt and Drought Tolerance in Arabidopsis.
Genes (Basel)
October 2022
Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China.
Sweet potato ( (L.) Lam) is one of the most crucial food crops widely cultivated worldwide. In plants, MYB transcription factors play crucial roles in plant growth, defense regulation, and stress resistance.
View Article and Find Full Text PDFWWP2 confers risk to osteoarthritis by affecting cartilage matrix deposition via hypoxia associated genes.
Osteoarthritis Cartilage
January 2023
Dept. of Biomedical Data Sciences, Section Molecular Epidemiology, Leiden University Medical Center, Leiden, the Netherlands. Electronic address:
Objective: To explore the co-expression network of the osteoarthritis (OA) risk gene WWP2 in articular cartilage and study cartilage characteristics when mimicking the effect of OA risk allele rs1052429-A on WWP2 expression in a human 3D in vitro model of cartilage.
Method: Co-expression behavior of WWP2 with genes expressed in lesioned OA articular cartilage (N = 35 samples) was explored. By applying lentiviral particle mediated WWP2 upregulation in 3D in vitro pellet cultures of human primary chondrocytes (N = 8 donors) the effects of upregulation on cartilage matrix deposition was evaluated.
A DNA vaccine targeting VEE virus delivered by needle-free jet-injection protects macaques against aerosol challenge.
NPJ Vaccines
April 2022
U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter St, Fort Detrick, MD, 21702, USA.
We have previously shown that DNA vaccines expressing codon optimized alphavirus envelope glycoprotein genes protect both mice and nonhuman primates from viral challenge when delivered by particle-mediated epidermal delivery (PMED) or intramuscular (IM) electroporation (EP). Another technology with fewer logistical drawbacks is disposable syringe jet injection (DSJI) devices developed by PharmaJet, Inc. These needle-free jet injection systems are spring-powered and capable of delivering vaccines either IM or into the dermis (ID).
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