AI Article Synopsis
- The study investigated Ig gene rearrangement in peritoneal cavity B-1 cells and its potential role in autoantibody production.
- Mice with a targeted integration of the gfp gene into the rag1 locus were created to analyze the expression of RAG1 and Ig gene rearrangement in these cells.
- Findings indicated that RAG1 expression is not observed in Pc B-1 cells of adolescent or elderly mice and is not induced by LPS stimulation, suggesting prolonged inhibition is crucial for maintaining allelic exclusion of Ig genes.
Article Abstract
It has been proposed that Ig gene rearrangement in the peritoneal cavity (Pc) B-1 cells might be involved in autoantibody generation. To study possible secondary B cell maturation, we prepared mice carrying a target integration of gfp gene into a rag1 locus (rag1/gfp mice). The GFP+ cells express rag1 mRNA and are undergoing Ig gene rearrangement. RAG1 expression was studied in Pc B-1 cells to detect cells during the stage of Ig gene rearrangement. In contrast to previous reports, Pc B-1 cells did not show RAG1 expression in adolescent or elderly mice. RAG1 expression was not induced in Pc B-1 cells in vivo after stimulation by oral or i.p. administration of LPS. Our results suggest that RAG1 expression in Pc B-1 cells is inhibited for a long period under normal condition and that this suppression is an essential state which maintains allelic exclusion of Ig genes.
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