AI Article Synopsis
- A new member of the hepatoma-derived growth factor (HDGF) family, called HRP-3, has been identified and cloned in humans and mice, featuring a conserved hath region and a lack of a secretion signal peptide.
- HRP-3 is predominantly expressed in the testis and brain, with lesser expression in other organs, and it possesses a putative nuclear localization signal indicating its function in the nucleus.
- Experimental evidence shows that HRP-3 enhances DNA synthesis in transfected cells, suggesting its role in cell proliferation, particularly in the brain, testis, and heart.
Article Abstract
Hepatoma-derived growth factor (HDGF) and HDGF-related proteins (HRP) belong to a gene family with a well-conserved amino acid sequence at the N-terminus (the hath region). A new member of the HDGF family in humans and mice was identified and cloned; we call it HRP-3. The deduced amino acid sequence from HRP-3 cDNA contained 203 amino acids without a signal peptide for secretion. HRP-3 has its 97-amino-acid sequence at the N-terminus, which is highly conserved with the hath region of the HDGF family proteins. It also has a putative bipartite nuclear localizing signal (NLS) sequence in a similar location in its self-specific region of HDGF and HRP-1. Northern blot analysis shows that HRP-3 is expressed predominantly in the testis and brain, to an intermediate extent in the heart, and to a slight extent in the ovaries, kidneys, spleen, and liver in humans. Transfection of green fluorescent protein (GFP)-tagged HRP-3 cDNA showed that HRP-3 translocated to the nucleus of 293 cells. GFP-HRP-3 transfectants significantly increased their DNA synthesis more than cells transfected with vector only. The HRP-3 gene was mapped to chromosome 15, region q25 by FISH analysis. These findings suggest that a new member of the HDGF gene family, HRP-3, may function mainly in the nucleus of the brain, testis, and heart, probably for cell proliferation.
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| http://dx.doi.org/10.1006/bbrc.1999.1733 | DOI Listing |
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