AI Article Synopsis
- Glycosphingolipids were extracted from pig lung and divided into two groups based on the number of sugar residues: one with 3 or fewer, and another with 3 or more.
- Various techniques, including gas chromatography and mass spectrometry, were employed to analyze the oligosaccharides derived from these glycolipid fractions.
- A new high-performance liquid chromatography method using "on-flow" proton NMR was developed for effective characterization and purification of the glycolipids, confirming the results obtained from traditional analytical methods.
Article Abstract
Glycosphingolipids were prepared from pig lung and pooled into two fractions with (i) < or = 3 sugar residues, and (ii) > or = 3 sugar residues. Oligosaccharides were prepared and used for gas chromatography, gas chromatography/mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry. The glycolipid fractions i and ii were further characterised and purified using a novel method based on high performance liquid chromatography "on-flow" proton nuclear magnetic resonance. The LC "on-flow" NMR technique showed good chromatographic separation and gave NMR spectral information which could be used as guidance for pooling of the separated mixture glycolipids. Conventional 1H NMR, thin layer immunostaining, gas chromatography, gas chromatography/mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry were used to characterise the glycolipids and to validate LC-NMR spectral data.
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| http://dx.doi.org/10.1023/a:1006901803636 | DOI Listing |
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