Aims: To demonstrate the value of environmental scanning electron microscopy (ESEM) when used in combination with immunogold/silver enhancement methods as a valuable tool in ocular research, and to determine the phenotype of macrophages associated with the tunica vasculosa lentis while maintaining a topographical view of the lens surface.
Methods: Prenatal and postnatal rat eyes were investigated by conventional scanning electron microscopy and ESEM. In the latter case tissues were prestained with a panel of antileucocyte monoclonal antibodies and visualised with colloidal gold conjugated secondary antibody followed by silver enhancement.
Results: The preliminary data demonstrate that ED1(+) ED2(+) macrophages occur in large numbers around the lens and are associated with sectors of both normal vessels and those undergoing regression.
Conclusion: The present study demonstrates that ESEM is an ideal way to combine scanning electron microscopy with immunohistochemistry and is therefore likely to have multiple other applications in ocular research.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1722901 | PMC |
| http://dx.doi.org/10.1136/bjo.83.12.1384 | DOI Listing |
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