Nucleoside diphosphate kinase (NDP kinase; ATP: NDP phosphotransferase; EC 2.7.4.6) was purified from bovine retina. The molecular mass of the native enzyme was found to be 72 kDa, and those of its subunits were 17.5 and 18.5 kDa. Kinetic characteristics of the enzyme were determined. It was shown that NDP kinase exists in retina in both soluble and membrane-bound forms.
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Biochemistry
December 2024
Gavin Herbert Eye Institute - Center for Translational Vision Research, Department of Ophthalmology, University of California, Irvine, Irvine, California 92697, United States.
Arrestins halt signal transduction by binding to the phosphorylated C-termini of activated G protein-coupled receptors. Arrestin-1, the first subtype discovered, binds to rhodopsin in rod cells. Mutations in , the gene encoding Arrestin-1, are linked to Oguchi disease, characterized by delayed dark adaptation.
View Article and Find Full Text PDFJ Biol Chem
December 2024
Center for Vision Research and the Department of Ophthalmology and Visual Sciences, SUNY Upstate Medical University, Syracuse, New York, USA. Electronic address:
Invest Ophthalmol Vis Sci
November 2024
Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, Qro., México.
Purpose: Growth hormone (GH) has neuroprotective effects that have not been evaluated in the mammalian visual system. This study tested the hypothesis that GH administration can promote retinal neuroprotection in an optic nerve crush (ONC) model in male rats.
Methods: The ON was compressed for 10 seconds, and bovine GH was injected concomitantly to injury for 14 days (0.
Pharmaceuticals (Basel)
October 2024
Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX 77004, USA.
We have evidence that hydrogen sulfide (HS)-releasing compounds can reduce intraocular pressure in normotensive and glaucomatous rabbits by increasing the aqueous humor (AH) outflow through the trabecular meshwork. Since HS has been reported to possess neuroprotective actions, the prevention of retinal ganglion cell loss is an important strategy in the pharmacotherapy of glaucoma. Consequently, the present study aimed to investigate the neuroprotective actions of HS-releasing compounds against hydrogen peroxide (HO)-induced oxidative stress in an isolated bovine retina.
View Article and Find Full Text PDFItal J Pediatr
October 2024
Pediatrics and Neonatology, Pediatric Department, Alexandria University Faculty of Medicine, Alexandria University, Alexandria, Egypt.
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