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Ecto-protein kinase CK2, the neglected form of CK2.
Biomed Rep
April 2018
Medical Biochemistry and Molecular Biology, Saarland University, D-66424 Homburg, Germany.
Ecto-protein kinases, including protein kinase CK2 (former name, casein kinase 2), have been the focus of research for more than 30 years. At the beginning of the ecto-kinase research their identification was performed with substrates and inhibitors whose specificity under the current knowledge was rather limited. Since all currently known ecto-kinases, including ecto-CK2, have intracellular counterparts, one has to exclude that an ecto-localization originates from intracellular counterparts after cell damage.
View Article and Find Full Text PDFIdentification of extracellularly phosphorylated membrane proteins.
Proteomics
October 2015
Department of Molecular Cardiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Ecto-protein kinases phosphorylate extracellular membrane proteins and exhibit similarities to casein kinases and protein kinases A and C. However, the identification of their protein substrates still remains a challenge because a clear separation from intracellular phosphoproteins is difficult. Here, we describe a straightforward method for the identification of extracellularly phosphorylated membrane proteins in human umbilical vein endothelial cells (HUVECs) and K562 cells which used the protease bromelain to selectively remove ectoproteins from intact cells and combined this with the subsequent analysis using IMAC and LC-MS/MS.
View Article and Find Full Text PDFEcto-protein kinases and phosphatases: an emerging field for translational medicine.
J Transl Med
June 2014
Department of Developmental Biology, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USA.
Progress in translational research has led to effective new treatments of a large number of diseases. Despite this progress, diseases including cancer and cardiovascular disorders still are at the top in death statistics and disorders such as osteoporosis and osteoarthritis represent an increasing disease burden in the aging population. Novel strategies in research are needed more than ever to overcome such diseases.
View Article and Find Full Text PDFAllosteric modulation of GABAA receptors by extracellular ATP.
Mol Brain
January 2014
Brain Research Centre and Department of Medicine, Vancouver Coastal Health Research Institute, University of British Columbia, Vancouver, BC V6T 2B5, Canada.
Background: The γ-aminobutyric acid type A receptor (GABAAR) is the primary receptor mediating fast synaptic inhibition in the brain and plays a critical role in modulation of neuronal excitability and neural networks. Previous studies have demonstrated that ATP and its nucleotide analogs may regulate the function of GABAARs via Ca2+-dependent intracellular mechanisms, which require activation of purinergic 2 (P2) receptors or cross-talk between two receptors.
Results: Here, we report a potentiation of GABAARs by extracellular ATP via a previously un-recognized allosteric mechanism.
Stimulation of Leishmania tropica protein kinase CK2 activities by platelet-activating factor (PAF).
Acta Trop
September 2009
Disciplina de Parasitologia, DMIP, FCM, Universidade do Estado do Rio de Janeiro, Rua Professor Manoel de Abreu, 444, PAPC 5 degrees andar, Vila Isabel, 20550-170, Rio de Janeiro, Brazil.
Leishmania tropica is one of the causative agents of cutaneous leishmaniasis. Platelet-activating factor (PAF) is a phospholipid mediator in diverse biological and pathophysiological processes. Here we show that PAF promoted a three-fold increase on ecto-protein kinase and a three-fold increase on the secreted kinase activity of L.
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