We have partially sequenced more than 1000 NotI linking clones isolated from human chromosome 3-specific libraries. Of these clones, 152 were unique chromosome 3-specific clones. The clones were precisely mapped using a combination of fluorescence in situ hybridization (FISH) and hybridization to somatic cell or radiation hybrids. Two- and three-color FISH was used to order the clones that mapped to the same chromosomal region, and in some cases, chromosome jumping was used to resolve ambiguous mapping. When this NotI restriction map was compared with the yeast artificial chromosome (YAC) based chromosome 3 map, significant differences in several chromosome 3 regions were observed. A search of the EMBL nucleotide database with these sequences revealed homologies (90-100%) to more than 100 different genes or expressed sequence tags (ESTs). Many of these homologies were used to map new genes to chromosome 3. These results suggest that sequencing NotI linking clones, and sequencing CpG islands in general, may complement the EST project and aid in the discovery of all human genes by sequencing random cDNAs. This method may also yield information that cannot be obtained by the EST project alone; namely, the identification of the 5' ends of genes, including potential promoter/enhancer regions and other regulatory sequences

Download full-text PDF

Source
http://dx.doi.org/10.1016/s0378-1119(99)00411-4DOI Listing

Publication Analysis

Top Keywords

noti linking
12
linking clones
12
clones isolated
8
isolated human
8
chromosome
8
human chromosome
8
chromosome 3-specific
8
est project
8
clones
7
analysis noti
4

Similar Publications

[Estimate of undiagnosed HIV cases in the Cajamarca region of Peru: probabilistic linkage between databases].

Cien Saude Colet

June 2023

Laboratorio de Referencia Nacional Virus de Transmisión Sexual VIH/SIDA, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima Perú.

Undiagnosed HIV cases contribute to the increase in new infections, therefore estimating this figure is important in order to assess strategies in HIV control programs. The objective of this study was to estimate the number of undiagnosed HIV cases in the Cajamarca region in Peru between 2015 and 2021. HIV cases were obtained from three sources of information: The Regional Health Strategy for HIV Prevention and Control (ESPC-HIV); the HIV epidemiological notification application (Noti-HIV), and the laboratory information system (Netlab).

View Article and Find Full Text PDF

Vibrio cholerae O1 El Tor, causative agent of the ongoing seventh cholera pandemic, is native to the aquatic environment of the Ganges Delta, Bay of Bengal (GDBB). Recent studies traced pandemic strains to the GDBB and proposed global spread of cholera had occurred via intercontinental transmission. In the research presented here, NotI-digested genomic DNA extracted from V.

View Article and Find Full Text PDF

Construction of a full-length antibody phage display vector.

J Immunol Methods

July 2021

Key Laboratory for Biological Medicine in Shandong Universities, Weifang Key Laboratory for Antibodies Medicine, School of Life Science and Technology, Weifang Medical University, Weifang 261053, China; World Research Hub Initiative (WRHI), Institute of Innovative Research, Tokyo Institute of Technology, Yokohama 226-8503, Japan. Electronic address:

Article Synopsis
  • Antibody phage display technology is crucial for creating monoclonal antibodies and enhancing their properties, but existing methods primarily display only parts of the antibody.
  • This study introduces a new phage display system capable of presenting full-length IgG antibodies by incorporating both heavy and light chains into the M13 phage vector.
  • The system demonstrates effective antibody expression in E. coli, retains the antibodies’ ability to bind to antigens, and offers a novel approach for constructing and screening antibody libraries.
View Article and Find Full Text PDF

In video games, identification with avatars-virtual entities or characters driven by human behavior-has been shown to serve many interpersonal and intraindividual functions (like social connection, self-expression, or identity exploration) but our understanding of the psychological variables that influence players' avatar choices remains incomplete. The study presented in this paper tested whether players' preferred style of avatar creation is linked to the magnitude of self-perceived discrepancies between who they are, who they aspire to be, and who they think they should be. One-hundred-and-twenty-five undergraduate gamers indicated their preferred avatar creation style and completed a values measure from three different perspectives: their actual, ideal, and ought selves.

View Article and Find Full Text PDF

Recurrent cholera causes significant morbidity and mortality in cholera endemic estuarine areas of Bangladesh. There have been limited studies to investigate the transmission patterns of associated with cholera in Bangladesh. In this study, we characterized serogroup O1 isolated from 30 cholera patients, 76 household contacts, 119 stored drinking water samples, and 119 water source samples in Bakerganj and Mathbaria, two cholera endemic coastal regions in Bangladesh.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!