Combination of paclitaxel and radiation: genotoxicity in vitro in four mammalian cell lines.

Paclitaxel is an antimicrotubular agent that blocks the cells in the G2/M phase of the cell cycle. Due to this action, it is presumed that this drug could function as a radiation sensitizer. We studied the genotoxic effects of a combination of paclitaxel and radiation in four mammalian cell lines in the micronucleus assay. The results do not show a clear radiation-sensitizing effect. In the three cell lines, L5178Y, V79 and HeLa, the micronucleus frequencies varied around a theoretical additive effect of the single treatments (paclitaxel or radiation alone). Only the human breast cancer cell line MCF-7 showed consistently lower than additive micronucleus frequencies, although the deviation was not significant. Overall, it remains inconclusive whether paclitaxel exerts a radiosensitizing effect and, if so, whether this effect depends on the cell type or other characteristics of tumor biology.