: An EST database of more than 900 sequences has been constructed from complementary DNAs from six different tissues (stomach, intestine, pyloric cecum, liver, spleen, and ovary) of the winter flounder Pleuronectes americanus. Template preparation and automated sequencing were optimized to generate high-quality information in an economic fashion. Using computer scripts developed in our laboratory, the sequences were automatically compared with sequences in the databases via a Web-browser interface, and significant returns were recorded and organized on user-friendly HTML pages. Half (453) of the ESTs had significant matches to database sequences of known function, 33 matched ESTs from other organisms, 34 matched ribosomal RNAs, and 24 matched hypothetical open reading frames of unknown function. Forty-one percent (374) of the ESTs had no matches to sequences in the database and presumably represent previously unidentified cDNAs. Several sequences are the first isolated from teleost fish, and should be of interest for gene mapping and studies of developmental biology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/pl00011802 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Relations between microplastic contamination and stress biomarkers under two seasonal conditions in wild carps, mullets and flounders.
Mar Environ Res
January 2025
ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Departamento de Estudos de Populações, Laboratório de Ecotoxicologia e Ecologia, (ECOTOX), Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal; CIIMAR / CIMAR-LA - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Research Team of Aquatic Ecotoxicology and One Health, and Research Team of Contaminant Pathways in Marine Environment, Terminal de Cruzeiros do Porto de Leixões, 4450-208, Matosinhos, Portugal. Electronic address:
Potential effects of microplastics (MP, plastic particles <5 mm) on the levels of multiple stress biomarkers were investigated in wild fish populations of Cyprinus carpio, Mugil cephalus, Platichthys flesus captured in the Minho River estuary located in the Iberian Peninsula. Specimens were collected in March and September 2018, corresponding to the end of winter and summer, respectively. Based on the concentration of MP determined by FT-IR analysis and morphological inspection, fishes from each species were divided into two groups: ≤0.
View Article and Find Full Text PDFEffect of larval rearing temperature on steroidogenesis pathway development in winter flounder (Pseudopleuronectes americanus) early life history.
Comp Biochem Physiol A Mol Integr Physiol
January 2025
Institut des sciences de la mer de Rimouski, Université du Québec à Rimouski, Rimouski, QC G5L 3A1, Canada. Electronic address:
Winter flounder (Pseudopleuronectes americanus) is a North Atlantic flatfish that inhabits cold-water environments already affected by global warming. Flatfishes are particularly sensitive during their juvenile stage to a phenomenon known as temperature-dependent sex determination (TSD). In this study, we hypothesized that many genes involved in the steroidogenesis pathway are already expressed at the larval stage in winter flounder and that temperature conditions may influence this pathway prior to the juvenile stage, which is usually considered the TSD-sensitive period.
View Article and Find Full Text PDFEmergent conformational and aggregation properties of synergistic antimicrobial peptide combinations.
Nanoscale
November 2024
Biological Physics & Soft Matter Group, Department of Physics, King's College London, London, WC2R 2LS, UK.
Synergy between antimicrobial peptides (AMPs) may be the key to their evolutionary success and could be exploited to develop more potent antibacterial agents. One of the factors thought to be essential for AMP potency is their conformational flexibility, but characterising the diverse conformational states of AMPs experimentally remains challenging. Here we introduce a method for characterising the conformational flexibility of AMPs and provide new insights into how the interplay between conformation and aggregation in synergistic AMP combinations yields emergent properties.
View Article and Find Full Text PDFDiverse Origins of Near-Identical Antifreeze Proteins in Unrelated Fish Lineages Provide Insights Into Evolutionary Mechanisms of New Gene Birth and Protein Sequence Convergence.
Mol Biol Evol
September 2024
Department of Biological Sciences, University of Arkansas, Fayetteville, AR, USA.
Determining the origins of novel genes and the mechanisms driving the emergence of new functions is challenging yet crucial for understanding evolutionary innovations. Recently evolved fish antifreeze proteins (AFPs) offer a unique opportunity to explore these processes, particularly the near-identical type I AFP (AFPI) found in four phylogenetically divergent fish taxa. This study tested the hypothesis of protein sequence convergence beyond functional convergence in three unrelated AFPI-bearing fish lineages.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!