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The development and application of a novel reagent for fixing red blood cells with glutaraldehyde and paraformaldehyde.
Hematology
December 2023
Department of Blood Transfusion, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.
Objective: The currently employed red blood cell reagents have a short shelf life. Some hospitals with a small number of specimens will be unable to utilize them within the validity period, resulting in a substantial increase in the purchase price. Therefore, the method of developing long-term red blood cell reagents is a problem worthy of further study.
View Article and Find Full Text PDFMALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration.
Methods Mol Biol
January 2022
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic.
In mass spectrometry imaging (MSI), the essential steps in sample preparation include collection and storage. The most widely used preservation procedure for MSI consists in freezing samples and storing them at temperatures below -80 °C. On the other hand, the most common method for preserving biological samples in clinical practice is their fixation in paraformaldehyde.
View Article and Find Full Text PDFMass spectrometry imaging (MSI) is a modern analytical technique capable of monitoring the spatial distribution of compounds within target tissues. Collection and storage are important steps in sample preparation. The recommended and most widely used preservation procedure for MSI is freezing samples in isopentane and storing them at temperatures below -80 °C.
View Article and Find Full Text PDF[Analysis of the storage time in paraformaldehyde-fixed T cells and their subsets in Kunming mice].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
April 2017
College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China. *Corresponding author, E-mail:
Objective To determine the expiration dates of CD3eCD4 T and CD3eCD8a T lymphocytes of Kunming mice. Methods The sterile spleen and anticoagulant blood of Kunming mice were collected and used to prepare the single-cell suspensions. After CD3e, CD4 and CD8a of T cells were stained with fluorophore-conjugated monoclonal antibodies, the samples were fixed with paraformaldehyde (PFA) and stored at 4DegreesCelsius in dark followed by detection using three-color flow cytometry at several specific time points.
View Article and Find Full Text PDFTargeted single-cell microchemical analysis: MS-based peptidomics of individual paraformaldehyde-fixed and immunolabeled neurons.
Chem Biol
August 2012
Department of Chemistry and the Beckman Institute for Advanced Science and Technology, University of Illinois Urbana-Champaign, Urbana, IL 61801, USA.
Pinpointing a specific cell from within a relatively uniform cell population to determine its chemical content presents a challenging bioanalytical task. Immunocytochemistry is the classical method used to localize specific molecules and, hence, selected cells. Mass spectrometry also probes endogenous molecules such as neuropeptides within a cell.
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