AI Article Synopsis
- Newly synthesized mammalian spermatozoa undergo essential changes in the epididymis, which enhance their ability to fertilize eggs due to specific gene expression in this region.
- Researchers identified a novel transcript, cy-ESP13.2, in cynomolgus monkeys that encodes a likely secretory protein related to similar proteins in humans.
- The study demonstrated that cy-ESP13.2 is highly expressed in specific parts of the epididymis and is associated with epithelial cells, but it is not found on the sperm cells themselves.
Article Abstract
Newly synthesized mammalian spermatozoa undergo critical modifications as they pass along the epididymis. The modifications endow spermatozoa with fertilizing ability and occur largely as a consequence of epididymal gene expression. With this in mind, we here employed a cDNA cloning strategy designed to identify key epididymal gene products. We describe a novel cynomolgus monkey (Macaca fascicularis) epididymal transcript designated cy-ESP13.2, of 690 nucleotides. The putative human ortholog was cloned and is highly conserved. Both cDNA sequences predict small, secretory proteins with a disulfide-stabilized core. Anti-peptide polyclonal antibodies were raised to a predicted cy-ESP13.2 surface loop. Western blotting with these antibodies revealed high-level, epididymis-specific expression of cy-ESP13.2, consistent with the pattern of cy-ESP13.2 mRNA expression assessed by Northern blotting. cy-ESP13.2 protein was of 30 kDa and was readily detectable in epithelial cells lining the efferent ductules, initial segment, and cauda regions of the epididymis, but not on spermatozoa. Similarities to members of the four-disulfide-core family suggest clues to ESP13.2 function.
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| http://dx.doi.org/10.1095/biolreprod61.4.965 | DOI Listing |
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