AI Article Synopsis
- Oxygen radicals can have both harmful and beneficial effects in biological systems, but they are hard to detect due to their fleeting nature and effective antioxidant defenses.
- In plants, oxygen is crucial for photosynthesis, where it is generated through water oxidation and used as an electron acceptor, making photosynthetic components vulnerable to oxygen radical damage.
- We present a new, efficient voltammetric method for measuring superoxide levels in vitro, showing that superoxide is produced not only by photosystem I but also by photosystem II, likely from the Q(A) site under reducing conditions.
Article Abstract
Oxygen radicals play both pathological and physiological roles in biological systems. The detection of such radicals is difficult due to their transient nature and the presence of highly efficient antioxidant mechanisms. In plants the physiological role of oxygen is twofold, oxygen is produced by the oxidation of water and consumed as an electron acceptor. The direct involvement of oxygen in photosynthetic events exposes the photosynthetic apparatus to a high probability of damage by oxygen radicals. We report here a direct, simple and rapid method for the measurement of superoxide in vitro based on voltammetric detection. It has potential applications for other in vitro systems investigating superoxide production. We show that in addition to the well established production of superoxide from photosystem I, under reducing conditions superoxide is also produced by photosystem II, probably from the Q(A) site.
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| http://dx.doi.org/10.1016/s0014-5793(99)01067-4 | DOI Listing |
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