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Sanger validation of WGS variants.
Sci Rep
January 2025
Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, Moscow, Russia, 125315.
With the development of next-generation sequencing (NGS) technologies it became possible to simultaneously analyze millions of variants. Despite the quality improvement, it is generally still required to confirm the variants before reporting. However, in recent years the dominant idea is that one could define the quality thresholds for "high quality" variants which do not require orthogonal validation.
View Article and Find Full Text PDFDistinct bacteria display genus and species-specific associations with mycobionts in paramo lichens in Colombia.
FEMS Microbiol Ecol
January 2025
Grupo de Max Planck Tándem en Biología Computacional y Ecología Microbiana, Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia.
Lichens are complex symbiotic systems where fungi interact with an extracellular arrangement of one or more photosynthetic partners and an indeterminate number of other microbes. Recently, specific lichen-microbial community associations have been proposed. In this study, we aimed to characterize the differences in bacteria associated with closely related lichens, under a defined set of environmental conditions in Colombian paramos.
View Article and Find Full Text PDFK1 channels enable myelinated axons to transmit spikes reliably without spiking ectopically.
J Neurosci
January 2025
Neurosciences and Mental Health, The Hospital for Sick Children, Toronto, Ontario, Canada
Action potentials (spikes) are regenerated at each node of Ranvier during saltatory transmission along a myelinated axon. The high density of voltage-gated sodium channels required by nodes to reliably transmit spikes increases the risk of ectopic spike generation in the axon. Here we show that ectopic spiking is avoided because K1 channels prevent nodes from responding to slow depolarization; instead, axons respond selectively to rapid depolarization because K1 channels implement a high-pass filter.
View Article and Find Full Text PDF[Measurement of Airborne Radioactivity in the Inpatient Room after Administering [Cu]Cu-ATSM to Patients].
Kaku Igaku
January 2025
Department of Radiology, Kindai University Faculty of Medicine.
Objective: [Cu]Cu-ATSM is a radiotherapeutics under clinical trials. It is necessary to take appropriate measures to limit its exposure and ensures its airborne concentrations do not exceed legally permitted levels. Therefore, the purpose of this study was to measure the airborne radioactivity concentration in the inpatient room after administering [Cu]Cu-ATSM to patients.
View Article and Find Full Text PDFA miniaturized colorimetric-photoacoustic dual-mode device for point-of-care testing of ascorbic acid and ascorbate oxidase.
Anal Chim Acta
March 2025
The Radiology Department of Shanxi Provincial People's Hospital Affiliated to Shanxi Medical University, Taiyuan, 030001, China. Electronic address:
Background: Real-time and rapid detection of ingredients in food has important significance for food safety. However, traditional detection methods not only require bulky and costly instruments but also are often based on single-mode analysis, limiting their accuracy and applications in point-of-care testing. Herein, an integrated and miniaturized dual-mode device based on colorimetric and photoacoustic (PA) principles is developed, using Au@Ag nanoparticles (Au@AgNPs) as signal probe and ascorbic acid (AA) and ascorbate oxidase (AAO) as analytes.
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