Objective And Design: We have developed a continuous monitoring system that will quantify lymphocyte extravasation or intercellular permeability with intracellular calcium measurements in a single preparation.
Materials: Human microvascular endothelial cells, human lymphocytes and histamine.
Treatment: Endothelial barrier function and intracellular calcium were examined upon application of either human lymphocytes (1 x 10(5) cells/ml) or histamine (1 microM).
Methods: Endothelial cells labeled with FURA-2 were examined for both changes in endothelial barrier function and mobilization of intracellular calcium using fluorescence spectroscopy and ratio imaging, respectively.
Results: Increases in endothelial intracellular calcium occur in the first minute and are followed by increases in endothelial intercellular permeability to albumin (5-7 min) or lymphocytes (15-20 min). Approximately 60 min following calcium mobilization, there was a second and larger extravasation.
Conclusion: The continuous monitoring system expands our understanding of the time course of the permeability and extravasation events to mobilization of endothelial intracellular calcium.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s000110050477 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biodegradable copper-doped calcium phosphate nanoplatform enables tumor microenvironment modulations for amplified ferroptosis in cervical carcinoma treatment.
Int J Pharm X
June 2025
Department of Gynecology, The Affiliated Hospital of Kunming University of Science and Technology, The First People's Hospital of Yunnan Province, Kunming, PR China.
As a recently discovered form of regulated cell death, ferroptosis has attracted much attention in the field cancer therapy. However, achieving considerably enhanced efficacy is often restricted by the overexpression of endogenous glutathione (GSH) in tumor microenvironment (TME). In this work, we report a ferroptosis-inducing strategy of GSH depletion and reactive oxygen species (ROS) generation based on a biodegradable copper-doped calcium phosphate (CaP) with L-buthionine sulfoximine (BSO) loading (denoted as BSO@CuCaP-LOD, BCCL).
View Article and Find Full Text PDFProtein palmitoylation is involved in regulating mouse sperm motility via the signals of calcium, protein tyrosine phosphorylation and reactive oxygen species.
Biol Res
January 2025
School of Pharmacy, Hangzhou Medical College, Hangzhou, Zhejiang, China.
Background: Protein palmitoylation, a critical posttranslational modification, plays an indispensable role in various cellular processes, including the regulation of protein stability, mediation of membrane fusion, facilitation of intracellular protein trafficking, and participation in cellular signaling pathways. It is also implicated in the pathogenesis of diseases, such as cancer, neurological disorders, inflammation, metabolic disorders, infections, and neurodegenerative diseases. However, its regulatory effects on sperm physiology, particularly motility, remain unclear.
View Article and Find Full Text PDFCPK1 activates CNGCs through phosphorylation for Ca signaling to promote root hair growth in Arabidopsis.
Nat Commun
January 2025
State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences (CAS), Shanghai, 200032, China.
Cyclic nucleotide-gated channel 5 (CNGC5), CNGC6, and CNGC9 (CNGC5/6/9 for simplicity) control Arabidopsis root hair (RH) growth by mediating the influx of external Ca to establish and maintain a sharp cytosolic Ca gradient at RH tips. However, the underlying mechanisms for the regulation of CNGCs remain unknown. We report here that calcium dependent protein kinase 1 (CPK1) directly activates CNGC5/6/9 to promote Arabidopsis RH growth.
View Article and Find Full Text PDFPKCα regulates the secretion of PDL1-carrying small extracellular vesicles in a p53-dependent manner.
Cell Death Dis
January 2025
School of Pharmacy, Faculty of Medicine & State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, China.
Small extracellular vesicles (sEVs), carrying PD-L1, have been implicated in immune evasion and tumor progression. However, understanding how PD-L1 sEVs are secreted still needs to be improved. We found that the secretion dynamics of PD-L1 sEVs is similar to that of other sEVs.
View Article and Find Full Text PDFThe malaria parasite PP1 phosphatase controls the initiation of the egress pathway of asexual blood-stages by regulating the rounding-up of the vacuole.
PLoS Pathog
January 2025
LPHI, UMR 5294 CNRS/UM-UA15 Inserm, Université de Montpellier, Montpellier, France.
A sustained blood-stage infection of the human malaria parasite P. falciparum relies on the active exit of merozoites from their host erythrocytes. During this process, named egress, the infected red blood cell undergoes sequential morphological events: the rounding-up of the surrounding parasitophorous vacuole, the disruption of the vacuole membrane and finally the rupture of the red blood cell membrane.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!