Immunization of mice with TNF alpha-activated porcine endothelial cells led to the characterization of two monoclonal antibodies (MAbs), 5F3 and 8A7, specific for porcine VCAM-1. Upon flow cytometry, both antibodies increasingly labeled endothelial cells according to their degree of activation. They bound a band of MW 80 kDa on Western blots of endothelial cells, which is the apparent molecular weight of porcine VCAM-1. It was determined by surface plasmon resonance that the antibodies are directed to different antigenic sites. It was also found that 5F3 competes for binding the antigen with a MAb previously characterized as binding domain 1 of porcine VCAM-1. Subsequently, 5F3, but not 8A7, was found to inhibit the adhesion of human B lymphocyte Ramos cells to porcine endothelial cells in vitro. These antibodies, which do not cross-react with human VCAM-1, might be useful for diagnostic or therapeutic purposes in xenotransplantation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1089/hyb.1999.18.159 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cytokine Levels in Experimental Branch Retinal Vein Occlusion Treated With Either Bevacizumab or Triamcinolone Acetonide.
Transl Vis Sci Technol
June 2024
Centre for Ophthalmology and Visual Science, The University of Western Australia, Perth, Western Australia, Australia.
Purpose: To compare gene expression changes following branch retinal vein occlusion (BRVO) in the pig with and without bevacizumab (BEV) and triamcinolone acetonide (TA).
Methods: Photothrombotic BRVOs were created in both eyes of four groups of nine pigs (2, 6, 10, and 20 days). In each group, six pigs received intravitreal injections of BEV in one eye and TA in the fellow eye, with three pigs serving as untreated BRVO controls.
Activated factor X stimulates atrial endothelial cells and tissues to promote remodelling responses through AT1R/NADPH oxidases/SGLT1/2.
Cardiovasc Res
September 2024
University of Strasbourg, UR 3074, Translational Cardiovascular Medicine, Biomedicine Research Center of Strasbourg, 1 Rue Eugène Boeckel, 67000 Strasbourg, France.
Article Synopsis
- Atrial fibrillation (AF) is linked to heart issues and involves changes in heart tissue; this study explores how activated factor X (FXa) impacts this process in atrial endothelial cells and human heart tissues.
- The researchers used cells from pig hearts and human surgical samples, testing for various proteins, gene expressions, and signs of stress and fibrosis in response to FXa.
- Findings showed that FXa boosts harmful responses like oxidative stress and inflammation in atrial cells while reducing protective factors, and these effects can be blocked by specific inhibitors targeting FXa.
Single-nucleus transcriptomics of epicardial adipose tissue from female pigs reveals effects of exercise training on resident innate and adaptive immune cells.
Cell Commun Signal
April 2024
Department of Veterinary Physiology and Pharmacology, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
Article Synopsis
- Coronary artery disease (CAD) significantly affects women, with epicardial adipose tissue (EAT) playing a dual role by providing beneficial energy while also contributing to CAD progression through inflammation if excessive.
- A study on female Yucatan pigs assessed the effects of chronic aerobic exercise on EAT, comparing sedentary and exercise groups while mimicking CAD through artery occlusion.
- Results indicated that exercise upregulated key cellular signaling pathways and enhanced interactions among immune, endothelial, and mesenchymal cells in EAT, potentially creating a more anti-inflammatory environment.
MicroRNA-155 mediates multiple gene regulations pertinent to the role of human adipose-derived mesenchymal stem cells in skin regeneration.
Front Bioeng Biotechnol
March 2024
Department of Hand Surgery, Plastic Surgery, and Burns, Linkoping University Hospital, Linköping, Sweden.
The role of Adipose-derived mesenchymal stem cells (AD-MSCs) in skin wound healing remains to be fully characterized. This study aims to evaluate the regenerative potential of autologous AD-MSCs in a non-healing porcine wound model, in addition to elucidate key miRNA-mediated epigenetic regulations that underlie the regenerative potential of AD-MSCs in wounds. The regenerative potential of autologous AD-MSCs was evaluated in porcine model using histopathology and spatial frequency domain imaging.
View Article and Find Full Text PDFExpression of genes regulating cell division in porcine follicular granulosa cells.
Cell Div
August 2023
Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, Torun, Poland.
Background: Cell cycle regulation influences the proliferation of granulosa cells and affects many processes related to ovarian folliclular growth and ovulation. Abnormal regulation of the cell cycle can lead to many diseases within the ovary. The aim of this study was to describe the expression profile of genes within granulosa cells, which are related to the formation of the cytoskeleton, organization of cell organelles inside the cell, and regulation of cell division.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!