The Saccharomyces cerevisiae gene PDC5 encodes the minor isoform of pyruvate decarboxylase (Pdc). In this work we show that expression of PDC5 but not that of PDC1, which encodes the major isoform, is repressed by thiamine. Hence, under thiamine limitation both PDC1 and PDC5 are expressed. PDC5 also becomes strongly expressed in a pdc1delta mutant. Two-dimensional gel electrophoresis of whole protein extracts shows that thiamine limitation stimulates the production of THI gene products and of Pdc5p. Deletion of PDC1 only stimulates production of Pdc5p. We conclude that the stimulation of PDC5 expression in a pdc1delta mutant is not due to a response to thiamine limitation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0014-5793(99)00449-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
TiCT MXene nanoribbons@MnO: A novel multifunctional probe for colorimetric and fluorescence dual-response sensing of trichlorfon.
Talanta
December 2024
Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China; College of Agricultural Equipment Engineering, Henan University of Science and Technology, Luoyang, 471003, China. Electronic address:
Manganese dioxide nanosheets (MnO NSs) have garnered significant attention in analytical sensing, while the majority of the previous reports suffer from a complex preparation process involving reducing agents, template or high-temperature. In this work, a novel MnO NSs decorated TiCT MXene nanoribbons (TiCTNR@MnO) composite was firstly assemblied via a facile one-step strategy and applied as a bi-signal generator to enable colorimetric and fluorescence (FL) dual-response sensing. During the assembly process, TiCTNR innovatively acted as both reductant and carrier to prevent the aggregation of MnO NSs.
View Article and Find Full Text PDFCluster Headache and Hypoxia: Breathing New Life into an Old Theory, with Novel Implications.
Neurol Int
December 2024
Department of Psychology, University of Maine, 301 Williams Hall, Orono, ME 04469-5742, USA.
Cluster headache is a severe, poorly understood disorder for which there are as yet virtually no rationally derived treatments. Here, Lee Kudrow's 1983 theory, that cluster headache is an overly zealous response to hypoxia, is updated according to current understandings of hypoxia detection, signaling, and sensitization. It is shown that the distinctive clinical characteristics of cluster headache (circadian timing of attacks and circannual patterning of bouts, autonomic symptoms, and agitation), risk factors (cigarette smoking; male gender), triggers (alcohol; nitroglycerin), genetic findings (GWAS studies), anatomical substrate (paraventricular nucleus of the hypothalamus, solitary tract nucleus/NTS, and trigeminal nucleus caudalis), neurochemical features (elevated levels of galectin-3, nitric oxide, tyramine, and tryptamine), and responsiveness to treatments (verapamil, lithium, melatonin, prednisone, oxygen, and histamine desensitization) can all be understood in terms of hypoxic signaling.
View Article and Find Full Text PDFUtilizing data from the Vitamin C, Thiamine, and Steroids in Sepsis (VICTAS) Trial, this hub model was developed to limit seventeen Renin-Angiotensin-Aldosterone System (RAAS) components as three entrance and four exits, to facilitate the calculation of a model as one egress unknown, the angiotensin type 1 (AT1) receptor. Following previous evidence relating renin levels to mortality, this study found controls were more like sepsis patients with levels < renin quartile 1 (Q1) for calculated AT1, while more like sepsis patients with renin levels > quartile 3 (Q4) for measured aldosterone levels. Additionally differential discrete correlate summation (DCS) analysis indicates AT1, aldosterone and renin as major hub nodes in this independent DCS model of metabolic data inputs.
View Article and Find Full Text PDFPhysiological traits, gene expression responses, and proteomics of rice varieties varying in heat stress tolerance at the flowering stage.
Front Plant Sci
December 2024
Guangxi Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China.
Introduction/background: Global warming greatly limits the productivity of rice. Rice plants are highly sensitive to heat stress at the flowering stage. The selection of heat-tolerant varieties is considered the most effective approach for ensuring global food security in the coming decades.
View Article and Find Full Text PDFIntegrated analysis of miRNA-mRNA regulatory networks in Anoectochilus roxburghii in response to blue laser light.
Tree Physiol
December 2024
Fujian Provincial Key Laboratory of Bamboo Resources Development and Utilization, Sanming University, No. 25 Jingdong, Sanming City, Fujian Province, 365004, China.
Anoectochilus roxburghii (Wall.) Lind. has significant medicinal and economic value, and the social demand for this species is increasing annually.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!