The sequence and genomic organization of the mouse Lim1 gene were determined. The mouse Lim1 gene has five coding exons. The Lim1 transcription initiation start site was determined by 5' RACE. indicating that the first exon encodes the translation initiation codon and a 1360-bp 5' untranslated region. Sequence analysis of the 450-bp upstream of the transcription start site revealed the presence of a CATTAA motif at -32 bp and a CAATT box located in reverse orientation at -68 bp. HNF3 beta and Pbx1 binding sites were also identified. Like most LIM domain encoding genes, the LIM domains of Lim1 are each encoded on separate and adjacent exons. Knowledge of the sequence and structure of the mouse Lim1 gene provides important information for the genetic manipulation of the Lim1 locus.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s003359901020 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chondrocyte Polarity During Endochondral Ossification Requires Protein-Protein Interactions Between Prickle1 and Dishevelled2/3.
J Bone Miner Res
December 2021
Department of Oral and Craniofacial Sciences, School of Dental Medicine, Center for Craniofacial Regeneration, University of Pittsburgh, Pittsburgh, PA, USA.
The expansion and growth of the endochondral skeleton requires organized cell behaviors that control chondrocyte maturation and oriented division. In other organs, these processes are accomplished through Wnt/planar cell polarity (Wnt/PCP) signaling pathway and require the protein-protein interactions of core components including Prickle1 (PK1) and Dishevelled (DVL). To determine the function of Wnt/PCP signaling in endochondral ossification of the cranial base and limb, we utilized the Prickle1 (Pk1 ) mouse line.
View Article and Find Full Text PDFStructural and functional analysis of LIM domain-dependent recruitment of paxillin to αvβ3 integrin-positive focal adhesions.
Commun Biol
March 2021
Department of Cell Physiology and Metabolism, University of Geneva, Centre Médical Universitaire, Geneva 4, Switzerland.
The LIM domain-dependent localization of the adapter protein paxillin to β3 integrin-positive focal adhesions (FAs) is not mechanistically understood. Here, by combining molecular biology, photoactivation and FA-isolation experiments, we demonstrate specific contributions of each LIM domain of paxillin and reveal multiple paxillin interactions in adhesion-complexes. Mutation of β3 integrin at a putative paxillin binding site (β3) leads to rapidly inward-sliding FAs, correlating with actin retrograde flow and enhanced paxillin dissociation kinetics.
View Article and Find Full Text PDFVariants of Focal Adhesion Scaffold Genes Cause Thoracic Aortic Aneurysm.
Circ Res
January 2021
Beijing Anzhen Hospital, Capital Medical University, China (Yang Li, S.G., Y.H., Y.K., Y.J., S.H., J.D., Yulin Li).
Rationale: Thoracic aortic aneurysm (TAA) leads to substantial mortality worldwide. Familial and syndromic TAAs are highly correlated with genetics. However, the incidence of sporadic isolated TAA (iTAA) is much higher, and the genetic contribution is not yet clear.
View Article and Find Full Text PDFThe cell biology and molecular genetics of Müllerian duct development.
Wiley Interdiscip Rev Dev Biol
May 2018
Monash Biomedicine Discovery Institute, Department of Anatomy and Development Biology, Monash University, Clayton, Victoria, Australia.
The Müllerian ducts are part of the embryonic urogenital system. They give rise to mature structures that serve a critical function in the transport and development of the oocyte and/or embryo. In most vertebrates, both sexes initially develop Müllerian ducts during embryogenesis, but they regress in males under the influence of testis-derived Anti-Müllerian Hormone (AMH).
View Article and Find Full Text PDFPolydiacetylenic nanofibers as new siRNA vehicles for in vitro and in vivo delivery.
Nanoscale
January 2018
V-SAT Laboratory, Vectors: Synthesis and Therapeutic Applications, Labex Medalis, CAMB UMR7199 CNRS-Université de Strasbourg, Faculty of Pharmacy, Illkirch, France.
Polydiacetylenic nanofibers (PDA-Nfs) obtained by photopolymerization of surfactant 1 were optimized for intracellular delivery of small interfering RNAs (siRNAs). PDA-Nfs/siRNA complexes efficiently silenced the oncogene Lim-1 in the renal cancer cells 786-O in vitro. Intraperitoneal injection of PDA-Nfs/siLim1 downregulated Lim-1 in subcutaneous tumor xenografts obtained with 786-O cells in nude mice.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!