The methods of deuterium-free water, as applied to the conditions of long-term space expeditions have been discussed. It is shown that with the use of the method of electrolysis of distilled water or urine condensate followed by the catalytic or high-temperature synthesis one can obtained water with four-fold decrease in deuterium concentration. In order to reduce the energy expenditure it is necessary to use the existing routine technologies of water and oxygen regeneration or the versions with applying the fuel cells in which hydrogen and oxygen resulting from electrolysis would generate the electrical power with simultaneous isolation of deuterium-free water.

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