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Dynamic and large field of view photonic resonator absorption microscopy for ultrasensitive digital resolution detection of nucleic acid and protein biomarkers.
Biosens Bioelectron
November 2024
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA; Nick Holonyak Jr. Micro and Nanotechnology Laboratory, Urbana, IL, 61801, USA; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA; Carl R. Woese Institute for Genomic Biology, Urbana, IL, 61801, USA; Cancer Center at Illinois, Urbana, IL, 61801, USA. Electronic address:
Article Synopsis
- - This paper presents an advanced biosensing instrument that enhances the previously developed photonic resonator absorption microscope (PRAM) by adding features like autofocus and digital tracking of gold nanoparticle (AuNP) movement during biomolecular assays.
- - The automated PRAM can capture wide panoramic images (“tiling”) and record dynamic sequences ("movies") to visualize and quantify the attachment and detachment of AuNPs on a photonic crystal surface.
- - The instrument is demonstrated in two assays for detecting protein biomarkers, achieving a sensitivity of detecting microRNA-375 at extremely low concentrations (1 aM) in a quick and enzyme-free process, highlighting its potential for use in point-of-care diagnostics and studying biomolecular interactions at the single
Is Seeing Believing? Hallmark Movie Viewership and Relationship Beliefs.
Psychol Rep
May 2024
Department of Psychology, University of Mississippi, University, MS, USA.
The Hallmark Channel, a highly watched cable network, is known for its consistently idealistic portrayals of romantic relationships. Despite its popularity, no research has examined whether increased viewership of Hallmark movies covaries with endorsement of relationship beliefs. According to cultivation theory, what we watch impacts our beliefs and perceptions of reality.
View Article and Find Full Text PDFRecording the direct surgeon's view with an operating microscopic view improves microscopic ophthalmic surgery training.
Int J Ophthalmol
September 2023
Ophthalmology, Osaka Red Cross Hospital, Tennoji-ku, Osaka 543-8555, Japan.
Aim: To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope, which lacks information on the movement and position of the surgeon's hands, and to facilitate the reproduction of a skilled surgeon's technique by a surgeon in training.
Methods: A small camera was attached to the operating microscope with a custom adapter. Microscopic surgeon's view and direct surgeon's view through this new camera were recorded in the surgical recording system.
3D-printed device for the kinetic determination of As(III) in groundwater samples by digital movie analysis.
Talanta
August 2023
INQUISUR (UNS-CONICET), Department of Chemistry, Universidad Nacional Del Sur, Av. Alem 1253, B8000CPB, Bahía Blanca, Argentina. Electronic address:
High concentrations of inorganic arsenic in groundwater for human consumption is a worldwide common problem. Particularly, the determination of As(III) becomes important, since this species is more toxic than organic, pentavalent and elemental arsenic forms. In this work, a 3D-printed device that included a 24-well microplate was developed to perform the colourimetric kinetic determination of arsenic (III) by digital movie analysis.
View Article and Find Full Text PDFApplying Blockchain Technology in Network Public Opinion Risk Management System in Big Data Environment.
Comput Intell Neurosci
March 2023
College of Movie and Media, Sichuan Normal University, Chengdu 610066, China.
Network public opinion represents public social opinion to a certain extent and has an important impact on formulating national policies and judgment. Therefore, China and other countries attach great importance to the study of online public opinion. However, the current researches lack the combination of theory and practical cases and lack the intersection of social and natural sciences.
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