A novel HLA-DR4 allele has been detected in a volunteer bone marrow donor using a reverse microtiter plate oligotyping assay. Exon 2 cloning and sequencing revealed the new HLA-DRB1*0431 allele which differs from DRB1*0408 by two nucleotide changes at codon 74 leading to an Ala/Leu substitution. Although typical of DR8 alleles, Leu74 polymorphism was not sufficient to confer any serological reactivity with DR8 alloantisera. This rare DR4 subtype was identified in an individual of East European origin. The HLA typing of this donor is: A2,A31; B18,B51; Cw*0701,Cw*15; DRB1*1602,DRB1*0431; DRB4*01,DRB5*02; DQB1*0301,DQB1*0502.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1034/j.1399-0039.1999.530409.x | DOI Listing |
Publication Analysis
Top Keywords
hla-drb1*0431 allele
8
sequence hla-drb1*0431
4
allele novel
4
novel hla-dr4
4
hla-dr4 allele
4
allele detected
4
detected volunteer
4
volunteer bone
4
bone marrow
4
marrow donor
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!